Barragán Verónica A, Aveiga Iván, Trueba Gabriel
Institute of Microbiology, University San Francisco de Quito, Quito, Ecuador.
Int Microbiol. 2008 Jun;11(2):121-6.
The microbial compositions of two soils from the northern Ecuadorian Amazon (Francisco de Orellana province), one contaminated with petroleum and the other uncontaminated, were compared. Classical culture and molecular techniques were used to analyze microbial diversity. The cultivable Bacteria from contaminated soil belonged to betaproteobacteria (16.6%), gammaproteobacteria (66.6%), and Firmicutes (16,6%), whereas in uncontaminated soil, cultivable Bacteria were identified as gammaproteobacteria (80%) and Firmicutes (20%). Analysis of the 16S rRNA showed that in the contaminated soil proteobacterial populations (alpha-, beta- and deltaproteobacteria) were more abundant than acidobacterial populations. The Shannon index (H cent ) was used to estimate diversity in the contaminated and uncontaminated soil. Diversity was higher in the uncontaminated (H cent = 2.16) than in the contaminated (H cent = 1.72) soil sample. Further studies are needed to determine whether the differences between contaminated and non-contaminated soil samples were due to spontaneous bioremediation microbial activity.
对来自厄瓜多尔亚马逊北部（弗朗西斯科·德·奥雷利亚纳省）的两种土壤的微生物组成进行了比较，其中一种被石油污染，另一种未受污染。采用传统培养和分子技术分析微生物多样性。受污染土壤中可培养的细菌属于β-变形菌门（16.6%）、γ-变形菌门（66.6%）和厚壁菌门（16.6%），而在未受污染的土壤中，可培养细菌被鉴定为γ-变形菌门（80%）和厚壁菌门（20%）。16S rRNA分析表明，在受污染土壤中，变形菌门菌群（α-、β-和δ-变形菌）比酸杆菌门菌群更为丰富。香农指数（H cent）用于估计受污染和未受污染土壤中的多样性。未受污染土壤（H cent = 2.16）的多样性高于受污染土壤（H cent = 1.72）样本。需要进一步研究以确定受污染和未受污染土壤样本之间的差异是否归因于自发生物修复微生物活性。
