长期石油污染土壤中细菌组成和多样性的特征分析及高效烷烃降解菌的分离（采用改良培养基）。
Characterization of bacterial composition and diversity in a long-term petroleum contaminated soil and isolation of high-efficiency alkane-degrading strains using an improved medium.
机构信息
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai, 200237, People's Republic of China.
出版信息
World J Microbiol Biotechnol. 2018 Feb 9;34(2):34. doi: 10.1007/s11274-018-2417-8.
Bacterial community and diversity in a long-term petroleum-contaminated soil of an oilfield were characterized using 16S rRNA gene-based Illumina MiSeq high-throughput sequencing. Results indicated that Proteobacteria (49.11%) and Actinobacteria (24.24%) were the most dominant phyla, and the most abundant genera were Pseudoxanthomonas (8.47%), Luteimonas (3.64%), Alkanindiges (9.76%), Acinetobacter (5.26%) and Agromyces (8.56%) in the soil. Meanwhile a series of cultivations were carried out for isolation of alkane degraders from petroleum-contaminated soil with gellan gum and agar as gelling agents. And the isolates were classified by their 16S rRNA genes. Nine of the isolates including Enterobacter, Pseudomonas,Acinetobacter, Rhizobium, Bacillus, Sphingomonas, Paenibacillus, Variovorax and Rhodococcus showed strong biodegradability of alkane mixture (C9-C30) in a wide range of chain-length, which could be potentially applied in enhancement of bioremediation.
采用基于 16S rRNA 基因的 Illumina MiSeq 高通量测序技术，对油田长期受石油污染土壤中的细菌群落结构和多样性进行了研究。结果表明，土壤中优势菌门为变形菌门（49.11%）和放线菌门（24.24%），优势菌属为假单胞菌属（8.47%）、黄单胞菌属（3.64%）、Alkanindiges 属（9.76%）、不动杆菌属（5.26%）和节杆菌属（8.56%）。同时，采用卡拉胶和琼脂作为凝胶剂，从石油污染土壤中进行了一系列的培养实验，以分离烷烃降解菌。并根据 16S rRNA 基因对分离株进行了分类。其中包括肠杆菌属、假单胞菌属、不动杆菌属、根瘤菌属、芽孢杆菌属、鞘氨醇单胞菌属、芽孢杆菌属、交替单胞菌属、沃氏菌属和红球菌属在内的 9 个分离株对烷烃混合物（C9-C30）具有较强的生物降解性，在较宽的链长范围内均具有较强的生物降解性，可潜在应用于生物修复的强化。
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