在培养的格林黑色素瘤细胞中，微管装配受到二丁酰腺苷3':5'-环磷酸或霍乱毒素的刺激。
Microtubule assembly in cultivated Greene melanoma cells is stimulated by dibutyryl adenosine 3':5'-cyclic monophosphate or cholera toxin.
作者信息
DiPasquale A M, McGuire J, Moellmann G, Wasserman S J
出版信息
J Cell Biol. 1976 Dec;71(3):735-48. doi: 10.1083/jcb.71.3.735.
Abstract
Both dibutyryl cyclic AMP (DBcAMP) and cholera toxin promote the formation and elongation of processes of cultivated Greene hamster melanoma cells. The formation and maintenance of these processes, which contain many microtubules, are sensitive to colcemid and vinblastine. Tubulin was measured by [3H]colchicine binding and by acrylamide gel electrophoresis. We found that DBcAMP or cholera toxin increases the ratio of polymerized to unpolymerized tubulin but not the total amount of tubulin per cell. The sum of the lengths of microtubules per unit area was significantly greater in cells treated with DBcAMP than in control cells. Our findings support the hypothesis that cyclic AMP promotes the elongation of cell processes by stimulating the assembly of microtubules from existing tubulin.
摘要
二丁酰环磷腺苷（DBcAMP）和霍乱毒素均可促进培养的格林仓鼠黑色素瘤细胞突起的形成和伸长。这些含有许多微管的突起的形成和维持对秋水仙酰胺和长春花碱敏感。通过[3H]秋水仙碱结合和丙烯酰胺凝胶电泳来测定微管蛋白。我们发现，DBcAMP或霍乱毒素可增加聚合态微管蛋白与未聚合态微管蛋白的比例，但不会增加每个细胞中微管蛋白的总量。与对照细胞相比，用DBcAMP处理的细胞中每单位面积微管的总长度明显更长。我们的研究结果支持这样的假说，即环磷腺苷通过刺激现有微管蛋白组装成微管来促进细胞突起的伸长。
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