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5-溴脱氧尿苷对组织培养中胎鼠肾上腺皮质细胞促肾上腺皮质激素依赖性线粒体生物合成的影响。

Effects of 5-bromodeoxyuridine on the ACTH-dependent mitochondrial biogenesis in cortical cells of fetal rat adrenals in tissue culture.

作者信息

Kahri A I, Salmenperä M, Saure A

出版信息

J Cell Biol. 1976 Dec;71(3):951-6. doi: 10.1083/jcb.71.3.951.

DOI:10.1083/jcb.71.3.951
PMID:186465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2109781/
Abstract

Cortical cells of fetal rat adrenals in tissue culture were treated with 5-bromodeoxyuridine (BrdU) during their proliferative phase and during ACTH stimulation when nuclear DNA synthesis has almost ceased. Pretreatment with 0.5 mug/ml/day of BrdU inhibited the ACTH-induced differentiation of cortical cells as well as the secretion of corticosterone and 18-OH-deoxycorticosterone (18-OHDOC). When nuclear DNA synthesis was suppressed and mitochondrial DNA synthesis was stimulated by ACTH BrdU addition (30 mug/ml/day) permitted normal untrastructural differentiation of cortical cells, except that the development of mitochondrial inner membranes was inhibited. Simultaneously mitochondrial inner membranes was inhibited. Simultaneously mitochondrial 11beta- and 18-hydroxylations were strongly inhibited while cytoplasmic 21-hydroxylation was not affected.

摘要

在组织培养中，对胎鼠肾上腺皮质细胞在其增殖期以及促肾上腺皮质激素（ACTH）刺激期间（此时核DNA合成几乎停止）用5-溴脱氧尿苷（BrdU）进行处理。每天用0.5微克/毫升的BrdU预处理会抑制ACTH诱导的皮质细胞分化以及皮质酮和18-羟基脱氧皮质酮（18-OHDOC）的分泌。当通过添加ACTH使核DNA合成受到抑制而线粒体DNA合成受到刺激时，添加BrdU（每天30微克/毫升）可使皮质细胞正常进行超微结构分化，只是线粒体内膜的发育受到抑制。同时，线粒体内膜受到抑制。同时，线粒体11β-和18-羟化作用受到强烈抑制，而细胞质21-羟化作用未受影响。

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Effects of 5-bromodeoxyuridine on the ACTH-dependent mitochondrial biogenesis in cortical cells of fetal rat adrenals in tissue culture.5-溴脱氧尿苷对组织培养中胎鼠肾上腺皮质细胞促肾上腺皮质激素依赖性线粒体生物合成的影响。
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引用本文的文献

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2
Effects of endogenous and exogenous cholesterol on the ultrastructure and steroid secretion of undifferentiated rat adrenocortical cells in primary culture.内源性和外源性胆固醇对原代培养的未分化大鼠肾上腺皮质细胞超微结构及类固醇分泌的影响。
Cell Tissue Res. 1990 Mar;259(3):421-7. doi: 10.1007/BF01740768.

本文引用的文献

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A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.一种用于电子显微镜的简化柠檬酸铅染色法。
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Inhibition by cycloheximide of ACTH-induced internal differentiation of mitochondria in cortical cells in tissue cultures of fetal rat adrenals.在胎鼠肾上腺组织培养中，放线菌酮对促肾上腺皮质激素诱导的皮质细胞线粒体内部分化的抑制作用。
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Selective inhibition by chloramphenicol of ACTH-induced reorganization of inner mitochondrial membranes in fetal adrenal cortical cells in tissue cultures.氯霉素对组织培养中胎儿肾上腺皮质细胞内促肾上腺皮质激素诱导的线粒体内膜重组的选择性抑制作用。
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The irreversible inhibition of differentiation of limb-bud mesenchyme by bromodeoxyuridine.溴脱氧尿苷对肢芽间充质分化的不可逆抑制作用。
Proc Natl Acad Sci U S A. 1972 May;69(5):1253-7. doi: 10.1073/pnas.69.5.1253.

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