Kahri A I, Salmenperä M, Saure A
J Cell Biol. 1976 Dec;71(3):951-6. doi: 10.1083/jcb.71.3.951.
Cortical cells of fetal rat adrenals in tissue culture were treated with 5-bromodeoxyuridine (BrdU) during their proliferative phase and during ACTH stimulation when nuclear DNA synthesis has almost ceased. Pretreatment with 0.5 mug/ml/day of BrdU inhibited the ACTH-induced differentiation of cortical cells as well as the secretion of corticosterone and 18-OH-deoxycorticosterone (18-OHDOC). When nuclear DNA synthesis was suppressed and mitochondrial DNA synthesis was stimulated by ACTH BrdU addition (30 mug/ml/day) permitted normal untrastructural differentiation of cortical cells, except that the development of mitochondrial inner membranes was inhibited. Simultaneously mitochondrial inner membranes was inhibited. Simultaneously mitochondrial 11beta- and 18-hydroxylations were strongly inhibited while cytoplasmic 21-hydroxylation was not affected.
在组织培养中，对胎鼠肾上腺皮质细胞在其增殖期以及促肾上腺皮质激素（ACTH）刺激期间（此时核DNA合成几乎停止）用5-溴脱氧尿苷（BrdU）进行处理。每天用0.5微克/毫升的BrdU预处理会抑制ACTH诱导的皮质细胞分化以及皮质酮和18-羟基脱氧皮质酮（18-OHDOC）的分泌。当通过添加ACTH使核DNA合成受到抑制而线粒体DNA合成受到刺激时，添加BrdU（每天30微克/毫升）可使皮质细胞正常进行超微结构分化，只是线粒体内膜的发育受到抑制。同时，线粒体内膜受到抑制。同时，线粒体11β-和18-羟化作用受到强烈抑制，而细胞质21-羟化作用未受影响。
