Salmenperä M, Kahri A I, Saure A
J Endocrinol. 1976 Aug;70(2):215-22. doi: 10.1677/joe.0.0700215.
The effects of corticosterone in concentrations found in adrenal venous plasma on ACTH-induced changes in cultured cortical cells derived from foetal rat adrenals were studied. Corticosterone at a concentration of 5-7 X 10(-5) mol/l completely inhibited mitochondrial differentiation to fasciculte-like morphology. The same cultures revealed significant inhibition of 11beta- and 18-hydroxylation compared with cultures treated with ACTH only. This was shown by the reduced formation of corticosterone and 18-OH-deoxycorticosterone (48%, P less than 0-001) and simultaneous enhancement of deoxycorticosterone formation (33%, P less than 0-05) from added [4-14C]progesterone. Similar inhibition was observed when dibutyryl cyclic AMP replaced ACTH as an inducer of differentiation. Lower concentrations of corticosterone (1-2 X 10(-5) and 2-4 X 10(-5) mol/l) inhibited ACTH-stimulated formation of corticosterone and 18-OH-deoxycorticosterone from endogenous precursors. The results demonstrate that corticosterone regulates the stage of differentiation in cultured adrenocortical cells. The possible role of corticosterone in the regulation of growth and steroidogenic capacity of the adrenal cortex is discussed.
研究了肾上腺静脉血浆中皮质酮浓度对促肾上腺皮质激素（ACTH）诱导的胎鼠肾上腺皮质细胞培养物变化的影响。浓度为5 - 7×10⁻⁵mol/L的皮质酮完全抑制了线粒体向束状样形态的分化。与仅用ACTH处理的培养物相比，相同培养物显示出11β-和18-羟化的显著抑制。这通过添加的[4-¹⁴C]孕酮生成皮质酮和18-羟基脱氧皮质酮的减少（48%，P < 0.001）以及脱氧皮质酮生成的同时增加（33%，P < 0.05）得以证明。当用二丁酰环磷腺苷替代ACTH作为分化诱导剂时，观察到类似的抑制作用。较低浓度的皮质酮（1 - 2×10⁻⁵和2 - 4×10⁻⁵mol/L）抑制了ACTH刺激的内源性前体生成皮质酮和18-羟基脱氧皮质酮。结果表明皮质酮调节培养的肾上腺皮质细胞的分化阶段。讨论了皮质酮在调节肾上腺皮质生长和类固醇生成能力中的可能作用。
