Hausman G J, Campion D R, Buonomo F C
Richard B. Russell Agricultural Research Center, USDA, Athens, GA 30613.
Growth Dev Aging. 1991 Spring;55(1):43-52.
An ontogeny study of lean and pre-obese pig fetuses was conducted to evaluate the temporal and genetic influence on the relationship between growth and IGF production. At 70, 90 and 110 days of gestation, three fetal pigs were obtained from each of three obese and lean dams. The heart, kidneys, lungs, liver and one biceps femoris muscle were removed, weighed, frozen and extracts prepared. For all the organ and tissue weights, there were significant (P less than .001) main effects of genotype (G) and fetal age (A). At each fetal age, pre-obese fetuses had lighter organ and tissue weights. The content of IGF's in serum and various tissues and/or organs was also determined. Main effects of A and G were significant (P less than .05) for liver and lung IGF-I and muscle IGF-II concentrations. Main effects of G were significant (P less than .01) for liver IGF-II whereas muscle IGF-I and lung IGF-II concentrations were only affected by fetal age (P less than .01). Overall, liver IGF-I and II and muscle IGF-II concentrations were higher in lean fetuses (P less than .01). Serum IGF-I concentration increased (P less than .01) with fetal age and was independent of fetal strain. Serum IGF-II concentration increased with age (P less than .01) in lean but not pre-obese fetuses. Therefore, these studies demonstrate genotype dependent growth factor concentrations in tissues and organs of developing fetuses. Finally, serum concentration of IGF's were strongly correlated (positive) with overall growth but were not strongly correlated with tissue and organ concentration of IGFs.
进行了一项关于瘦型和肥胖前期猪胎儿个体发育的研究，以评估生长与胰岛素样生长因子（IGF）产生之间关系的时间和遗传影响。在妊娠70、90和110天时，从三只肥胖母猪和三只瘦型母猪中各获取三只胎儿猪。取出心脏、肾脏、肺、肝脏和一块股二头肌，称重、冷冻并制备提取物。对于所有器官和组织的重量，基因型（G）和胎龄（A）均有显著（P<0.001）的主效应。在每个胎龄，肥胖前期胎儿的器官和组织重量较轻。还测定了血清以及各种组织和/或器官中IGF的含量。对于肝脏和肺中IGF-I以及肌肉中IGF-II的浓度，A和G有显著（P<0.05）的主效应。对于肝脏IGF-II，G有显著（P<0.01）的主效应，而肌肉IGF-I和肺IGF-II的浓度仅受胎龄影响（P<0.01）。总体而言，瘦型胎儿肝脏中IGF-I和II以及肌肉中IGF-II的浓度较高（P<0.01）。血清IGF-I浓度随胎龄增加（P<0.01），且与胎儿品系无关。瘦型胎儿血清IGF-II浓度随年龄增加（P<0.01），而肥胖前期胎儿则不然。因此，这些研究表明发育中胎儿的组织和器官中生长因子浓度具有基因型依赖性。最后，血清中IGF的浓度与总体生长呈强正相关，但与组织和器官中IGF的浓度相关性不强。
