Peng M, Pelletier G, Palin M F, Véronneau S, LeBel D, Abribat T
Agriculture and Agri-Food Canada Dairy and Swine Research and Development Centre, Québec, Canada.
Growth Dev Aging. 1996 Autumn-Winter;60(3-4):171-87.
As far as we know, there is no available information about ontogenic changes of tissue concentrations of IGF-I and II and IGFBPs in large mammals. Serum, liver and kidney levels of IGFs and IGFBPs were examined in fetuses at 90 and 110 days of gestation and in pigs at 1d, 3 wk, 3 mo and 6 mo of age. Ontogeny of mRNA levels of IGFs, IGF type I and type II receptors (IGFI-R and IGFII-R), IGFBP-1 and -3 (IGFBPs) and growth hormone receptor (GHR) were also examined by Northern blot analysis in liver, kidney and skeletal muscle of pig. Serum IGF-I, IGF-II and IGFBP-3 concentrations were low during the fetal life and increased after birth. The highest level of IGF-II mRNA was found in fetuses for all studied tissues. In the liver, IGF-I mRNA level and its protein content peaked at 3 wk of age. The highest IGF-II concentration was found at 1d and 3 wk of age. The IGFII-R mRNA remained at a constant level during the whole development period. The most abundant IGFBP-1 mRNA and its protein content were found at birth. The level of IGFBP-2 was high during fetal and early postnatal life. The IGFBP-3 content was relatively low in fetuses and reached the highest level after 3 wk of age. In the kidney, IGFs, IGFBP-3, IGFI-R and IGFII-R as well as GHR mRNA levels were relatively high during the fetal and early postnatal life. The IGFs concentrations were the highest in newborns. In the skeletal muscle, IGFs, IGFBP-3 and IGFI-R mRNA levels decreased with advancing age. During the postnatal life, the high IGFs concentrations in the liver and the kidney correspond to fast growth periods of these organs.
据我们所知，关于大型哺乳动物中胰岛素样生长因子-I（IGF-I）、胰岛素样生长因子-II（IGF-II）以及胰岛素样生长因子结合蛋白（IGFBPs）组织浓度的个体发生变化尚无可用信息。对妊娠90天和110天的胎儿以及1日龄、3周龄、3月龄和6月龄的猪的血清、肝脏和肾脏中的IGFs和IGFBPs水平进行了检测。还通过Northern印迹分析检测了猪的肝脏、肾脏和骨骼肌中IGFs、I型和II型胰岛素样生长因子受体（IGFI-R和IGFII-R）、IGFBP-1和-3（IGFBPs）以及生长激素受体（GHR）的mRNA水平个体发生情况。胎儿期血清IGF-I、IGF-II和IGFBP-3浓度较低，出生后升高。在所有研究组织中，胎儿期的IGF-II mRNA水平最高。在肝脏中，IGF-I mRNA水平及其蛋白质含量在3周龄时达到峰值。IGF-II浓度在1日龄和3周龄时最高。IGFII-R mRNA在整个发育期间保持恒定水平。出生时发现最丰富的IGFBP-1 mRNA及其蛋白质含量。IGFBP-2水平在胎儿期和出生后早期较高。胎儿期IGFBP-3含量相对较低，3周龄后达到最高水平。在肾脏中，胎儿期和出生后早期IGFs、IGFBP-3、IGFI-R和IGFII-R以及GHR mRNA水平相对较高。新生儿的IGFs浓度最高。在骨骼肌中，IGFs、IGFBP-3和IGFI-R mRNA水平随年龄增长而降低。在出生后的生命过程中，肝脏和肾脏中较高的IGFs浓度与这些器官的快速生长时期相对应。
