Chen Ailiang, Luo Mingyong, Yuan Guohua, Yu Jian, Deng Tuo, Zhang Liang, Zhou Yuxiang, Mitchelson Keith, Cheng Jing
Medical Systems Biology Research Center, Tsinghua University School of Medicine, Beijing, 100084, Peoples' Republic of China.
Biotechnol Lett. 2008 Dec;30(12):2045-52. doi: 10.1007/s10529-008-9800-8. Epub 2008 Jul 22.
MicroRNAs (miRNAs) and mRNAs constitute an important part of gene regulatory networks, influencing diverse biological phenomena. To discover novel regulatory pathways during myeloid differentiation, we performed miRNA as well as mRNA expression profiling of in vitro-differentiating HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). The main findings were up-regulation of miR-146a/b, miR-21, miR-221, miR-222, miR-155, miR-26a and down-regulation of miR-199a*, miR-181c, miR-142-3p, miR-92. After integrating the miRNA and mRNA expression data into a Transcriptome Interaction Database by Molecule Annotation System (MAS) software, a number of differently expressed mRNAs were revealed as potential targets of these miRNAs.
微小RNA（miRNA）和信使核糖核酸（mRNA）构成基因调控网络的重要部分，影响着多种生物学现象。为了发现髓系分化过程中的新调控途径，我们对用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）处理的体外分化HL - 60细胞进行了miRNA以及mRNA表达谱分析。主要发现为miR - 146a/b、miR - 21、miR - 221、miR - 222、miR - 155、miR - 26a上调，miR - 199a*、miR - 181c、miR - 142 - 3p、miR - 92下调。通过分子注释系统（MAS）软件将miRNA和mRNA表达数据整合到转录组相互作用数据库后，发现许多差异表达的mRNA是这些miRNA的潜在靶标。
