Rastegar M, Izadpanah K, Masumi M, Siampour M, Zare A, Afsharifar A
Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran.
Virus Genes. 2008 Oct;37(2):212-7. doi: 10.1007/s11262-008-0252-y. Epub 2008 Jul 22.
The complete nucleotide sequence of the genome of wheat Eqlid mosaic virus (WEqMV) (excluding the poly A tail) comprised 9636 nucleotides including 5' and 3' noncoding regions of 137 and 172 nt, respectively. It contained a single ORF coding for a polyprotein of 3,109 amino acid residues and had a deduced genome organization typical of members of the family Potyviridae and with proteinase cleavage sites very similar to those of the members of the genus Tritimovirus. Pairwise and multiple alignments and phylogenetic analysis showed that WEqMV is a distinct species in the genus Tritimovirus. WEqMV and Wheat streak mosaic virus (WSMV) shared the greatest nucleotide sequence identity in the NIb and HC-Pro cistrons (63.2% and 60.8%, respectively) and the lowest sequence identity in the P1 and CP cistrons (51.2% and 51.1%, respectively). Sequence identity for the complete genome of WEqMV and WSMV was 56.8% at the nucleotide level and 50.7% at the amino acid level. WEqMV had 57.2% nucleotide identity and 50.6% amino acid identity with Oat necrotic mottle virus and 52.5% nucleotide identity and 45.5% amino acid identity with Brome streak mosaic virus. The relationship of WEqMV with other members of the family Potyviridae was more distant. Structural analysis of WEqMV protein showed presence of potential transmembrane helices in 6k1, 6k2, and P3 proteins.
小麦伊克立德花叶病毒（WEqMV）基因组的完整核苷酸序列（不包括聚腺苷酸尾）由9636个核苷酸组成，包括分别为137和172个核苷酸的5'和3'非编码区。它包含一个编码3109个氨基酸残基多聚蛋白的单一开放阅读框，并且具有典型的马铃薯Y病毒科成员的推导基因组结构，其蛋白酶切割位点与小麦线条花叶病毒属成员的非常相似。成对和多重比对以及系统发育分析表明，WEqMV是小麦线条花叶病毒属中的一个独特物种。WEqMV和小麦条纹花叶病毒（WSMV）在NIb和HC-Pro顺反子中具有最高的核苷酸序列同一性（分别为63.2%和60.8%），而在P1和CP顺反子中具有最低的序列同一性（分别为51.2%和51.1%）。WEqMV和WSMV完整基因组在核苷酸水平上的序列同一性为56.8%，在氨基酸水平上为50.7%。WEqMV与燕麦坏死斑驳病毒的核苷酸同一性为57.2%，氨基酸同一性为50.6%；与雀麦条纹花叶病毒的核苷酸同一性为52.5%，氨基酸同一性为45.5%。WEqMV与马铃薯Y病毒科其他成员的关系更远。WEqMV蛋白的结构分析表明，在6k1、6k2和P3蛋白中存在潜在的跨膜螺旋。
