一种用于在定量力-位移测量过程中对单细胞反应进行成像的单轴生物微机电系统装置。

A uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements.

作者信息

Serrell David B, Law Jera, Slifka Andrew J, Mahajan Roop L, Finch Dudley S

机构信息

National Institute of Standards and Technology, Boulder, CO, USA.

University of Colorado, Boulder, CO, USA.

出版信息

Biomed Microdevices. 2008 Dec;10(6):883. doi: 10.1007/s10544-008-9202-7.

DOI:10.1007/s10544-008-9202-7

PMID:18648937

Abstract

A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied displacement. The device works in a fashion similar to that of a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless atomic force microscope (AFM) cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged by use of epifluorescence and phase contrast techniques.

摘要

一种微制造装置已被开发出来，用于在施加位移时对单个贴壁细胞进行成像并量化力。该装置的工作方式类似于位移控制的单轴拉伸机。该装置使用无尖原子力显微镜（AFM）悬臂进行校准，并且与计算出的弹簧常数显示出极好的一致性。对单个贴壁成纤维细胞施加阶跃输入，并使用力学模型对粘弹性响应进行表征。通过落射荧光和相差技术对贴壁成纤维细胞进行成像。