Muramatsu H, Chiba N, Umemoto T, Homma K, Nakajima K, Ataka T, Ohta S, Kusumi A, Fujihira M
Research Laboratory for Advanced Technology, Seiko Instruments, Inc. Chiba, Japan.
Ultramicroscopy. 1995 Dec;61(1-4):265-9. doi: 10.1016/0304-3991(95)00113-1.
This paper reports improvements of optical fiber cantilevers and the scanning near-field optical microscopy imaging of biological materials in liquid. In our scanning near-field optical/atomic-force microscope (SNOAM), the scanning of an optical fiber cantilever over the specimen was controlled by dynamic mode AFM to reduce damage to the probe and soft specimens. The typical resonant frequency of the optical fiber cantilever was 19.5 kHz, while it was 23.0 kHz in air. The Q-factor of the cantilever depended on the vibration amplitude and was typically 260-600 in air and 40-240 in water. The relationship between the vibration amplitude and the average sample-probe separation indicated that the cantilever worked in the non-contact mode in water, while it worked in the cyclic-contact mode in air. Cultured cells in aqueous solutions were visualized by the SNOAM, indicating that the SNOAM is suitable to observe soft specimens.
本文报道了光纤悬臂的改进以及在液体中对生物材料的扫描近场光学显微镜成像。在我们的扫描近场光学/原子力显微镜（SNOAM）中，通过动态模式原子力显微镜控制光纤悬臂在样品上的扫描，以减少对探针和软样品的损伤。光纤悬臂的典型共振频率为19.5千赫兹，而在空气中为23.0千赫兹。悬臂的品质因数取决于振动幅度，在空气中通常为260 - 600，在水中为40 - 240。振动幅度与平均样品 - 探针间距之间的关系表明，悬臂在水中以非接触模式工作，而在空气中以循环接触模式工作。通过SNOAM可以观察到水溶液中的培养细胞，这表明SNOAM适用于观察软样品。
