Gonzales Michelle, Saviola Beatrice
Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Mol Biol Rep. 2009 Jul;36(6):1225-9. doi: 10.1007/s11033-008-9301-9. Epub 2008 Jul 22.
The promoter driving expression of the Mycobacterium tuberculosis gene lipF (Rv3487c) had previously been identified as being upregulated by acidic stress. Subsequently a 59 base pair (bp) acid inducible minimal promoter region was isolated in which a putative -10 region was identified. In this study we use mutational analysis to investigate the -10 region of the lipF promoter. Mutations within this region lead to a dramatic decrease of promoter activity, while a mutation outside of this region does not affect promoter activity.
此前已确定驱动结核分枝杆菌基因lipF（Rv3487c）表达的启动子受酸性应激上调。随后分离出一个59个碱基对（bp）的酸诱导最小启动子区域，其中鉴定出一个推定的-10区域。在本研究中，我们使用突变分析来研究lipF启动子的-10区域。该区域内的突变导致启动子活性显著降低，而该区域外的突变不影响启动子活性。
