Richter Laetitia, Saviola Beatrice
Western University of Health Sciences, Pomona, CA 91766, USA.
Microbiol Res. 2009;164(2):228-32. doi: 10.1016/j.micres.2007.06.003. Epub 2007 Sep 14.
The lipF gene of Mycobacterium tuberculosis has been implicated in pathogenesis and its promoter has been shown to be upregulated by acidic stress. To further define the acidic pH that upregulates the lipF promoter from M. tuberculosis and to establish that it is specifically upregulated by acid stress and not by other environmental stresses, promoter expression levels were measured under a variety of conditions. The conditions measured were pH, temperature, oxidative stress, and hypoxic stress.
结核分枝杆菌的lipF基因与发病机制有关，其启动子已被证明在酸性应激下会上调。为了进一步确定上调结核分枝杆菌lipF启动子的酸性pH值，并确定它是由酸应激而非其他环境应激特异性上调的，在各种条件下测量了启动子的表达水平。所测量的条件包括pH值、温度、氧化应激和缺氧应激。
