Li Changqing, Grobmyer Stephen R, Massol Nicole, Liang Xiaoping, Zhang Qizhi, Chen Lin, Fajardo Laurie L, Jiang Huabei
Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611-6131, USA.
Med Phys. 2008 Jun;35(6):2493-501. doi: 10.1118/1.2921129.
This article presents a pilot study of multispectral diffuse optical tomography for noninvasively imaging volume fraction and mean size of cellular scattering components in the breast. Cellular morphology images for a total of 14 cases (four malignant breast and ten benign lesions) were obtained. Analyzing the images based on the pathological findings of the cases studied, we found that light scattering in the breast was contributed from both the nucleus and organelles such as mitochondria and nucleolus. Based on the image analysis of these 14 cases, we found that the differences in the mean size and volume fraction between the malignant and benign lesions are significant. The contrast ratio of the average mean size and volume fraction between malignant and benign lesions were calculated to be 3.38 and 2.63. These initial results suggest that cellular mean size and volume fraction may be two new criteria that could be used to differentiate malignant from benign lesions.
本文介绍了一项关于多光谱漫射光学层析成像的初步研究，用于对乳腺中细胞散射成分的体积分数和平均大小进行无创成像。共获得了14例病例（4例乳腺恶性肿瘤和10例良性病变）的细胞形态图像。根据所研究病例的病理结果对图像进行分析，我们发现乳腺中的光散射来自细胞核以及线粒体和核仁等细胞器。基于对这14例病例的图像分析，我们发现恶性和良性病变之间在平均大小和体积分数上的差异具有显著性。计算得出恶性和良性病变之间平均大小和体积分数的对比度分别为3.38和2.63。这些初步结果表明，细胞平均大小和体积分数可能是用于区分恶性和良性病变的两个新标准。
