Li Quan-Xia, Fan Bing-Quan, Gong Ming-Bo, Yang Hui
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Huan Jing Ke Xue. 2008 Mar;29(3):763-8.
A bacterial strain M11 using phenanthrene, anthracene, fluorethene,pyrene as a sole carbon and energy sources was isolated from soils contaminated with polycyclic aromatic hydrocarbons. Strain M11 was identified as Mycobacterium sp. according to the results of morphology, physiology and the phylogenetical analyses of 16S rDNA sequence. The degradation rate of pyrene (at levels of 50 mg/L, 100 mg/L and 200 mg/L) by strain M11 was 76.9%, 91.8% and 79.23% within 16 d in broth assay. Mycobacterium sp. M11 showed a strong ability to grow from pH 5 to pH 9 in liquid minimal medium containing pyrene 100 mg/L. Mycobacterium sp. M11 possessed aromatic-ring dioxygenase genes, which are highly homologous to the known nidAB genes from pyrene-degrading Mycobacteria.
从受多环芳烃污染的土壤中分离出一株以菲、蒽、芴、芘作为唯一碳源和能源的细菌菌株M11。根据形态学、生理学结果以及16S rDNA序列的系统发育分析，菌株M11被鉴定为分枝杆菌属。在肉汤试验中，菌株M11对芘（浓度分别为50mg/L、100mg/L和200mg/L）的降解率在16天内分别为76.9%、91.8%和79.23%。分枝杆菌属M11在含有100mg/L芘的液体基本培养基中，在pH值从5到9的范围内表现出很强的生长能力。分枝杆菌属M11拥有芳香环双加氧酶基因，这些基因与已知的芘降解分枝杆菌的nidAB基因高度同源。
