Zhang Qiao-qiao, Zhao Ye-jun, Yang Chao-guang, Liu Fen-wu, He Jian, Shen Biao, Ran Wei
Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Ying Yong Sheng Tai Xue Bao. 2010 Jul;21(7):1851-8.
Using pyrene as the sole carbon and energy source, and by the method of plate sublimation, a strain SE12 was isolated from a contaminated soil near Woniushan Coking Plant in Xuzhou, China. According to the morphological, biochemical, and 16S rDNA analyses, this strain was identified as Mycobacterium sp., with 98% of homology to the rapid-growth nonpathogenic strain M. austroafricanum ATCC 33464. The optimum pH and temperature for the degradation of pyrene by SE12 were pH 9 and 30 degrees C. When the soil samples were added with 100 and 200 mg x kg(-1) of pyrene and inoculated with 10(7) CFU x g(-1) of SE12, the degradation rates of pyrene reached to 97% and 99%, respectively after 28 days incubation at 30 degrees C. By using primer-pairs nidAF/nidAR and nidBF/nidBR for amplification of ring-hydroxylating dioxygenase genes, it was shown that SE12 had the fragments of encoded large and small subunits of dioxygenase genes. Sequence analysis showed that these fragments were highly homologous to the known dioxygenase genes from pyrene-degrading Mycobacteria sp.
以芘作为唯一的碳源和能源，采用平板升华法，从中国徐州卧牛山焦化厂附近的污染土壤中分离出一株SE12菌株。通过形态学、生化及16S rDNA分析，该菌株被鉴定为分枝杆菌属，与快速生长的非致病菌株南非分枝杆菌ATCC 33464具有98%的同源性。SE12降解芘的最适pH值和温度分别为pH 9和30℃。当向土壤样品中添加100和200 mg·kg⁻¹的芘，并接种10⁷ CFU·g⁻¹的SE12时，在30℃培养28天后，芘的降解率分别达到97%和99%。利用引物对nidAF/nidAR和nidBF/nidBR扩增环羟基化双加氧酶基因，结果表明SE12具有双加氧酶基因编码的大亚基和小亚基片段。序列分析表明，这些片段与已知的芘降解分枝杆菌属双加氧酶基因高度同源。
