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使用滤纸采集的标本进行幽门螺杆菌血清学检测。

Helicobacter pylori serology using specimens collected on filter paper.

作者信息

Huang Z, Dwyer B, Kaldor J

机构信息

Jiangsu Province Hospital, Nanjing, People's Republic of China.

出版信息

J Clin Pathol. 1991 Feb;44(2):167-9. doi: 10.1136/jcp.44.2.167.

DOI:10.1136/jcp.44.2.167
PMID:1864992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC496984/
Abstract

A simple method for the collection of blood specimens on filter paper for serology of Helicobacter pylori was devised. Blood was freshly applied to small Whatman 3 mm filter paper and allowed to dry. Serological activity, as measured by reactivity to H pylori antigens (AMRAD, Australia), was retained after storage for one month at both room temperature and at 4 degrees C. This technique eliminates formal venepuncture, separation of sera, and arduous packaging of specimens to be sent to the laboratory.

摘要

设计了一种用于幽门螺杆菌血清学检测的滤纸血样采集简单方法。将新鲜血液滴加到Whatman 3毫米小滤纸上，使其干燥。通过对幽门螺杆菌抗原的反应性（澳大利亚AMRAD公司）测定的血清学活性，在室温及4℃下储存一个月后仍能保持。该技术无需进行正规静脉穿刺、血清分离以及将标本送往实验室的繁琐包装。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74e3/496984/961e5768a493/jclinpath00404-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74e3/496984/961e5768a493/jclinpath00404-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74e3/496984/961e5768a493/jclinpath00404-0080-a.jpg

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引用本文的文献

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J Clin Pathol. 1999 Sep;52(9):633-9. doi: 10.1136/jcp.52.9.633.
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Diagnosis of rickettsial diseases using samples dried on blotting paper.使用吸水纸上干燥的样本诊断立克次氏体病。
Clin Diagn Lab Immunol. 1999 Jul;6(4):483-8. doi: 10.1128/CDLI.6.4.483-488.1999.
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Multi-laboratory comparison of eight commercially available Helicobacter pylori serology kits. Helicobacter pylori Serology Study Group.八种市售幽门螺杆菌血清学检测试剂盒的多实验室比较。幽门螺杆菌血清学研究组。

本文引用的文献

1
A SIMPLE PHENYLALANINE METHOD FOR DETECTING PHENYLKETONURIA IN LARGE POPULATIONS OF NEWBORN INFANTS.一种用于在大量新生儿群体中检测苯丙酮尿症的简易苯丙氨酸方法。
Pediatrics. 1963 Sep;32:338-43.
2
Detection of markers of hepatitis B infection in serum dried on to filter-paper: an application to field studies.滤纸干血斑中乙型肝炎感染标志物的检测：在现场研究中的应用
Bull World Health Organ. 1982;60(5):783-7.
3
Whole blood samples dried and stored on filter paper as substrate for the electrophoretic separation on hemoglobin S from hemoglobin A. A screening procedure.
Eur J Clin Microbiol Infect Dis. 1995 May;14(5):428-33. doi: 10.1007/BF02114899.
将全血样本干燥并储存在滤纸上，作为从血红蛋白A中电泳分离血红蛋白S的底物。一种筛查方法。
Clin Chim Acta. 1971 Nov;35(1):237-8. doi: 10.1016/0009-8981(71)90317-2.
4
Detecting antibodies to human immunodeficiency virus in dried blood on filter papers.检测滤纸上干血中的人类免疫缺陷病毒抗体。
J Infect Dis. 1987 May;155(5):1073-4. doi: 10.1093/infdis/155.5.1073.
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Too few hospital places for mentally abnormal offenders: plea for action by the Lord Chief Justice.为精神异常罪犯提供的医院床位过少：首席大法官呼吁采取行动。
Lancet. 1985 Feb 2;1(8423):269. doi: 10.1016/s0140-6736(85)91040-2.
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Storage of cerebrospinal fluid on paper.脑脊液在纸张上的存储。
Lancet. 1989 Oct 28;2(8670):1046. doi: 10.1016/s0140-6736(89)91064-7.