Zhuang H, Coulepis A G, Locarnini S A, Gust I D
Bull World Health Organ. 1982;60(5):783-7.
In an attempt to find a cheap, reliable, and convenient method for the transportation and storage of serum specimens during seroepidemiological surveys, a technique in which serum was dried on to pieces of filter paper was developed and evaluated. For the evaluation, a total of 382 sera were selected from the extensive serum collection held by the WHO Collaborating Centre for Virus Reference and Research at Fairfield Hospital, Australia. These sera were dried on to pieces of filter-paper, stored at different temperatures and then tested for the presence of the various markers of infection with hepatitis B virus by solid-phase radioimmunoassay. The results were in complete agreement with those obtained on whole serum specimens. In addition, storage at 4 degrees C, room temperature (22 degrees C), or 37 degrees C for up to 30 days did not alter the sensitivity of the test. This technique may be useful in field surveys, not only for the detection of hepatitis B infection, but also in the study of other diseases and metabolic disorders.
为了在血清流行病学调查中找到一种廉价、可靠且便捷的血清标本运输和储存方法，人们开发并评估了一种将血清干燥在滤纸片上的技术。为进行评估，从澳大利亚费尔菲尔德医院世界卫生组织病毒参考与研究合作中心保存的大量血清样本中总共选取了382份血清。这些血清被干燥在滤纸片上，在不同温度下储存，然后通过固相放射免疫测定法检测乙型肝炎病毒各种感染标志物的存在情况。结果与全血清标本检测结果完全一致。此外，在4℃、室温（22℃）或37℃下储存长达30天并未改变检测的灵敏度。该技术可能在现场调查中有用，不仅可用于检测乙型肝炎感染，还可用于其他疾病和代谢紊乱的研究。
