Hartwig Christina, Constabel Hanne, Neumann Detlef, Gerd Hoymann Heinz, Tschernig Thomas, Behrens Georg M N
Functional and Applied Anatomy, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
Exp Toxicol Pathol. 2008 Sep;60(6):425-34. doi: 10.1016/j.etp.2008.05.010. Epub 2008 Jul 22.
Murine models assist in elucidating the pathogenesis of allergic asthma and evaluation of new therapeutic strategies. We aimed to assess the requirement of boostering needed in the BL/6 murine asthma model and its influence on DC populations in lungs and bronchial lymph nodes.
Two injections of OVA+alum - one sensitization and one booster - followed by two aerosol challenges were sufficient to induce a distinct asthma-like inflammation in BL/6 mice, including significant increased immunoglobulin (IgE) level, influx of eosinophils in the airway lumen, and evident histopathology. Using this protocol, CD11chighMHC-II+ DC counts in lungs and lymph nodes doubled with no changes of CD8+ DC in the lungs but increase in lung-draining lymph nodes.
Given the site-specific changes of dendritic cell (DC) subpopulations during allergic asthma we propose a distinct regulation of antigen transport and antigen presentation in the murine asthma model.
小鼠模型有助于阐明过敏性哮喘的发病机制并评估新的治疗策略。我们旨在评估BL/6小鼠哮喘模型中加强免疫的必要性及其对肺和支气管淋巴结中树突状细胞（DC）群体的影响。
两次注射卵清蛋白（OVA）+明矾——一次致敏和一次加强免疫——随后进行两次雾化激发，足以在BL/6小鼠中诱导出明显的哮喘样炎症，包括免疫球蛋白（IgE）水平显著升高、气道腔内嗜酸性粒细胞浸润以及明显的组织病理学变化。采用该方案，肺和淋巴结中CD11c高表达MHC-II+ DC数量翻倍，肺中CD8+ DC数量无变化，但引流肺的淋巴结中CD8+ DC数量增加。
鉴于过敏性哮喘期间树突状细胞（DC）亚群的位点特异性变化，我们提出在小鼠哮喘模型中抗原转运和抗原呈递存在独特的调节机制。
