Institute of Anatomy and Cell Biology, Saarland University, Campus Homburg, Kirrberger Straße 100, 66424 Homburg, Germany.
Institute of Pharmacology, Medical School of Hannover, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
Int J Genomics. 2015;2015:638032. doi: 10.1155/2015/638032. Epub 2015 Feb 24.
Asthma is the consequence of allergic inflammation in the lung compartments and lung-draining lymph nodes. Dendritic cells initiate and promote T cell response and drive it to immunity or allergy. However, their modes of action during asthma are poorly understood. In this study, an allergic inflammation with ovalbumin was induced in 38 mice versus 42 control animals. After ovalbumin aerosol challenge, conventional dendritic cells (CD11c/MHCII/CD8) were isolated from the lungs and the draining lymph nodes by means of magnetic cell sorting followed by fluorescence-activated cell sorting. A comparative transcriptional analysis was performed using gene arrays. In general, many transcripts are up- and downregulated in the CD8(-) dendritic cells of the allergic inflamed lung tissue, whereas few genes are regulated in CD8(+) dendritic cells. The dendritic cells of the lymph nodes also showed minor transcriptional changes. The data support the relevance of the CD8(-) conventional dendritic cells but do not exclude distinct functions of the small population of CD8(+) dendritic cells, such as cross presentation of external antigen. So far, this is the first approach performing gene arrays in dendritic cells obtained from lung tissue and lung-draining lymph nodes of asthmatic-like mice.
哮喘是肺部和引流淋巴结的过敏炎症的结果。树突状细胞启动并促进 T 细胞反应，并将其驱动为免疫或过敏。然而，它们在哮喘中的作用模式知之甚少。在这项研究中，用卵清蛋白在 38 只小鼠和 42 只对照动物中诱导过敏炎症。卵清蛋白雾化挑战后，通过磁细胞分选和荧光激活细胞分选从肺部和引流淋巴结中分离出常规树突状细胞（CD11c/MHCII/CD8）。使用基因芯片进行了比较转录分析。一般来说，过敏炎症肺部组织中的 CD8(-)树突状细胞中许多转录物上调和下调，而 CD8(+)树突状细胞中很少有基因受到调节。淋巴结中的树突状细胞也表现出较小的转录变化。这些数据支持 CD8(-)常规树突状细胞的相关性，但不排除 CD8(+)树突状细胞的小群体的独特功能，例如外部抗原的交叉呈递。到目前为止，这是首次在类似哮喘的小鼠的肺部组织和引流淋巴结中获得的树突状细胞中进行基因芯片的方法。
