On-site manipulation of single chromosomal DNA molecules by using optically driven microstructures.
作者信息
Terao Kyohei, Washizu Masao, Oana Hidehiro
机构信息
Department of Micro Engineering, Kyoto University, Kyoto, 606-8501, Japan.
出版信息
Lab Chip. 2008 Aug;8(8):1280-4. doi: 10.1039/b803753a. Epub 2008 Jun 23.
DOI:10.1039/b803753a
PMID:18651069
Abstract
We report a novel method for manipulation of single giant DNA molecules under a video microscope. Using optically driven microstructures, we manipulated chromosomal DNA of length in the order of millimetres, extended by electroosmotic flow without DNA breakage in aqueous solution: we picked up DNA, using microfabricated hooks and wound it around microfabricated bobbins.
摘要
Zotero 插件