塔宾曲霉在木糖发酵中的乙醇耐受性。
The ethanol tolerance of Pachysolen tannophilus in fermentation on xylose.
机构信息
Beijing University of Chemical Technology, China.
出版信息
Appl Biochem Biotechnol. 2010 Jan;160(2):378-85. doi: 10.1007/s12010-008-8308-y. Epub 2008 Jul 24.
The influence of ethanol on fermentation by Pachysolen tannophilus was studied. When xylose utilization rate was 80%, ethanol concentration began to decline. Fermentation of P. tannophilus was affected by ethanol addition in the beginning of fermentation; average xylose consumption rate was 0.065 g.l(-1).h(-1), and maximum specific growth rate was 0.07 h(-1) at 28 g.l(-1) ethanol, comparing with the average xylose consumption rate of 0.38 g.l(-1).h(-1) and maximum specific growth rate of 0.14 h(-1) in fermentation with no ethanol addition; P. tannophilus stopped growth at 40 g.l(-1) ethanol. When the initial ethanol concentration was 30 g.l(-1), the addition of glucose in xylose media made the growth of P. tannophilus better, and the most favorable glucose concentration was 15 g.l(-1) with the highest biomass of 1.51 g.l(-1) as compared with that of 0.95 g.l(-1) in pure xylose media.
研究了乙醇对皱褶假丝酵母发酵的影响。当木糖利用率达到 80%时，乙醇浓度开始下降。乙醇的添加在发酵初期会影响皱褶假丝酵母的发酵；在 28g/L 乙醇下，平均木糖消耗率为 0.065g/L·h-1，最大比生长速率为 0.07h-1，而在无乙醇添加的发酵中，平均木糖消耗率为 0.38g/L·h-1，最大比生长速率为 0.14h-1；在 40g/L 乙醇下，皱褶假丝酵母停止生长。当初始乙醇浓度为 30g/L 时，在木糖培养基中添加葡萄糖使皱褶假丝酵母的生长状况更好，最有利的葡萄糖浓度为 15g/L，生物量最高为 1.51g/L，而在纯木糖培养基中为 0.95g/L。
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