Wiegand Annette, Buchholz Katharina, Werner Carola, Attin Thomas
Clinic for Preventive Dentistry, Periodontology and Cariology, University of Zürich, Zürich, Switzerland.
J Adhes Dent. 2008 Jun;10(3):227-32.
To evaluate the cytotoxicity of three desensitizers, one nonrinse, and one etch-and-rinse adhesive system applied on dentin specimens of different thickness.
The test materials (A: Admira Protect, B: Gluma Desensitizer, C: Seal&Protect, D: Clearfil Protect Bond, E: Optibond FL) and a positive control (35% H2O2) were applied on 1.0-, 1.5-, and 2.5-mm-thick bovine dentin specimens (each subgroup n = 5) in a dentin barrier test device. The experiments were performed with perfusion (2 ml/h) of the pulpal part of the chamber. The eluates were obtained before (baseline) and 15, 30, 45, 60, and 120 min after application of the adhesives and pipetted onto L-929 fibroblasts. Cytotoxicity of the materials was determined in relation to the baseline value using the MTT assay (succinic dehydrogenase activity). Statistical analysis was performed using ANOVA and Student's t-test.
With regard to 1.0-mm dentin specimens, application of Clearfil Protect Bond (D) decreased enzyme activity significantly, while test materials A to C and E were not cytotoxic. However, cytotoxicity of D was limited to up to 15 min and decreased thereafter. Application of the test materials A to E on 1.5- and 2.5-mm dentin samples exhibited no significant cytotoxic effects within 120 min. Generally, ANOVA found significant interactions between the test materials and dentin thickness. However, only for Admira Protect was a significant increase of enzyme activity with increasing dentin thickness observed.
Desensitizing agents might exhibit cytotoxic potential when applied on dentin less than 1.0 mm thick. The tested desensitizers and the adhesive systems caused similar effects, in which cytotoxicty might be influenced by the duration of perfusion and dentin thickness.
评估三种脱敏剂、一种免冲洗和一种酸蚀冲洗粘结系统应用于不同厚度牙本质标本时的细胞毒性。
将测试材料（A：Admira Protect、B：Gluma脱敏剂、C：Seal&Protect、D：Clearfil Protect Bond、E：Optibond FL）和一个阳性对照（35%过氧化氢）应用于牙本质屏障测试装置中1.0毫米、1.5毫米和2.5毫米厚的牛牙本质标本（每个亚组n = 5）。实验在腔室牙髓部分灌注（2毫升/小时）的情况下进行。在施加粘结剂之前（基线）以及之后15、30、45、60和120分钟获取洗脱液，并吸取到L-929成纤维细胞上。使用MTT法（琥珀酸脱氢酶活性）相对于基线值测定材料的细胞毒性。使用方差分析和学生t检验进行统计分析。
对于1.0毫米厚的牙本质标本，应用Clearfil Protect Bond（D）显著降低了酶活性，而测试材料A至C和E没有细胞毒性。然而，D的细胞毒性仅限于15分钟以内，之后降低。测试材料A至E应用于1.5毫米和2.5毫米厚的牙本质样本在120分钟内未表现出显著的细胞毒性作用。一般来说，方差分析发现测试材料和牙本质厚度之间存在显著相互作用。然而，仅对于Admira Protect观察到随着牙本质厚度增加酶活性显著增加。
当应用于厚度小于1.0毫米的牙本质时，脱敏剂可能表现出细胞毒性潜力。所测试的脱敏剂和粘结系统产生了相似的效果，其中细胞毒性可能受灌注持续时间和牙本质厚度的影响。
