Jiang R D, Lin H, Zheng G, Zhang X M, Du Q, Yang M
Dental Medical Devices Testing Center, Dental Materials Laboratory, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
Dental Medical Devices Testing Center, Dental Materials Laboratory, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
J Dent. 2017 Mar;58:28-33. doi: 10.1016/j.jdent.2017.01.003. Epub 2017 Jan 8.
To investigate the cytotoxicity of four dental restorative materials in three-dimensional (3D) L929 cell cultures using a dentin barrier test.
The cytotoxicities of light-cured glass ionomer cement (Vitrebond), total-etching adhesive (GLUMA Bond5), and two self-etching adhesives (GLUMA Self Etch and Single Bond Universal) were evaluated. The permeabilities of human dentin disks with thicknesses of 300, 500, and 1000μm were standardized using a hydraulic device. Test materials and controls were applied to the occlusal side of human dentin disks. The 3D-cell scaffolds were placed beneath the dentin disks. After a 24-h contact with the dentin barrier test device, cell viabilities were measured by performing MTT assays. Statistical analysis was performed using the Mann-Whitney U test.
The mean (SD) permeabilities of the 300-μm, 500-μm, and 1000-μm dentin disks were 0.626 (0.214), 0.219 (0.0387) and 0.089 (0.028) μlmincmcm HO. Vitrebond was severely cytotoxic, reducing the cell viability to 10% (300-μm disk), 17% (500μm), and 18% (1000μm). GLUMA Bond5 reduced the cell viability to 40% (300μm), 83% (500μm), and 86% (1000μm), showing moderate cytotoxicity (300-μm) and non-cytotoxicity (500-μm and 1000-μm). Single Bond Universal and GLUMA Self Etch did not significantly reduce cell viability, regardless of the dentin thicknesses, which characterized them as non-cytotoxic.
Cytotoxicity varied with the materials tested and the thicknesses of the dentin disks.
The tested cytotoxicity of materials applied on 300-, 500-, and 1000-μm dentin disks indicates that the clinical use of the test materials (excepting self-etching adhesives) in deep cavities poses a potential risk of damage to the pulp tissues to an extent, depending on the thickness of the remaining dentin.
使用牙本质屏障试验研究四种牙科修复材料在三维（3D）L929细胞培养中的细胞毒性。
评估光固化玻璃离子水门汀（Vitrebond）、全酸蚀粘结剂（GLUMA Bond5）和两种自酸蚀粘结剂（GLUMA Self Etch和Single Bond Universal）的细胞毒性。使用液压装置对厚度为300、500和1000μm的人牙本质圆盘的渗透性进行标准化。将测试材料和对照物应用于人牙本质圆盘的咬合面。将3D细胞支架放置在牙本质圆盘下方。与牙本质屏障试验装置接触24小时后，通过MTT试验测量细胞活力。使用Mann-Whitney U检验进行统计分析。
300μm、500μm和1000μm牙本质圆盘的平均（标准差）渗透率分别为0.626（0.214）、0.219（0.0387）和0.089（0.028）μlmincmcm HO。Vitrebond具有严重的细胞毒性，使细胞活力降低至10%（300μm圆盘）、17%（500μm）和18%（1000μm）。GLUMA Bond5使细胞活力降低至40%（300μm）、83%（500μm）和86%（1000μm），显示出中度细胞毒性（300μm）和无细胞毒性（500μm和1000μm）。无论牙本质厚度如何，Single Bond Universal和GLUMA Self Etch均未显著降低细胞活力，这表明它们无细胞毒性。
细胞毒性随测试材料和牙本质圆盘厚度的不同而变化。
在300μm、500μm和1000μm牙本质圆盘上测试的材料的细胞毒性表明，测试材料（自酸蚀粘结剂除外）在深龋中的临床应用在一定程度上对牙髓组织有潜在损害风险，这取决于剩余牙本质的厚度。
