Su Yong, Wen Yao, Zhu Weiyun
Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China.
Wei Sheng Wu Xue Bao. 2008 May;48(5):577-82.
To identify Lactobacillus sp. strain S1 from the intestine of piglet, and compare the genomic difference between this strain and Lactobacillus sobrius 001T.
Analysis of 16S rRNA gene sequence and species-specific polymerase chain reaction assay were used to identify Lactobacillus sp. strain S1. Representational difference analysis (RDA) was used to compare these two strains.
The 16S rRNA gene sequence analysis of strain S1 showed that its closest known species in the GenBank database was L. sobrius. Denaturing gradient gel electrophoresis analysis showed that the predominant bands in profiles from bacteria in the jejunum and ileum of piglets had the identical migrating position with strain S1. Cloning and sequencing of 16S rRNA gene analysis revealed that this band matched clone (Clone S) was also related closely to L. sobrius. Phylogenetic analysis of 16S rRNA gene showed that homology between strain S1 and Clone S was 99.8%, and that between strain S1 and L. sobrius was 99.6%. Both strains could be detected in 1.2% agarose gel by L. sobrius-specific PCR assay. Recently, RDA has been adapted to study the genomic diversity of bacterial strains. This analysis showed that there was no genomic difference between the two strains.
Piglet-derived strain S1 belonged to L. sobrius. Piglet-derived L. sobrius 001T and L. sobrius S1 were the similar strain.
从仔猪肠道中鉴定出乳酸杆菌属菌株S1，并比较该菌株与嗜酒乳酸杆菌001T之间的基因组差异。
采用16S rRNA基因序列分析和种特异性聚合酶链反应试验鉴定乳酸杆菌属菌株S1。运用代表性差异分析（RDA）比较这两个菌株。
菌株S1的16S rRNA基因序列分析表明，其在GenBank数据库中最相近的已知物种为嗜酒乳酸杆菌。变性梯度凝胶电泳分析显示，仔猪空肠和回肠中细菌图谱的主要条带与菌株S1具有相同的迁移位置。16S rRNA基因分析的克隆和测序表明，该条带匹配克隆（克隆S）也与嗜酒乳酸杆菌密切相关。16S rRNA基因的系统发育分析表明，菌株S1与克隆S之间的同源性为99.8%，菌株S1与嗜酒乳酸杆菌之间的同源性为99.6%。通过嗜酒乳酸杆菌特异性PCR试验，在1.2%琼脂糖凝胶中均可检测到这两种菌株。最近，RDA已被用于研究细菌菌株的基因组多样性。该分析表明这两个菌株之间没有基因组差异。
仔猪来源的菌株S1属于嗜酒乳酸杆菌。仔猪来源的嗜酒乳酸杆菌001T和嗜酒乳酸杆菌S1是相似菌株。
