Eisenbeiss W F, Apfel H, Meyer T F
Max-Planck-Institut für Biologie, Abteilung Infektionsbiologie, Tübingen, Federal Republic of Germany.
J Parasitol. 1991 Aug;77(4):580-6.
Living third- and fourth-stage larvae (L3 and L4) of Acanthocheilonema viteae were recovered quantitatively from adult Meriones unguiculatus within the first 10 days after subcutaneous inoculation of 60 arthropod-derived larvae (mL3). The average recovery of the inoculated larvae was about one third (28.5%), and the majority (87.7%) were found in muscular tissues. Seventy-two hours after inoculation, larvae could be isolated from all body locations, although the majority still was found near the site of inoculation. Morphological and biometrical data indicated that, at least until molting, the development of the larval population was not synchronous, with molting occurring over a period of 48 hr on days 7 and 8 postinoculation. The stomatal rings of postinvasive L3's and L4's were distinguishable structurally and could be used as stage-specific determinants. Immediately after infection, L3's showed a linear growth in diameter; rapid longitudinal growth started after the molt, leading to a doubling in the length of L4's within 4 days. The time course of shedding was reconstructed in detail using isolated L3/L4 intermediates.
在皮下接种60只节肢动物源幼虫（mL3）后的前10天内，定量回收了来自成年长爪沙鼠体内的棘唇丝虫活的第三和第四期幼虫（L3和L4）。接种幼虫的平均回收率约为三分之一（28.5%），且大部分（87.7%）发现于肌肉组织中。接种后72小时，尽管大部分幼虫仍在接种部位附近被发现，但在身体各个部位均可分离到幼虫。形态学和生物测量数据表明，至少在蜕皮前，幼虫群体的发育是不同步的，接种后第7天和第8天的48小时内会发生蜕皮。侵袭后L3和L4的气孔环在结构上是可区分的，可作为阶段特异性的决定因素。感染后，L3的直径呈线性增长；蜕皮后开始快速纵向生长，导致L4在4天内长度增加一倍。使用分离的L3/L4中间阶段详细重建了蜕皮的时间进程。
