Acanthocheilonema viteae: rational design of the life cycle to increase production of parasite material using less experimental animals.

The maintenance of the life cycle of Acanthocheilonema viteae is described with the aim to increase the production of parasite material using less experimental animals. The filaria was maintained in jirds (Meriones unguiculatus) and in soft ticks (Ornithodoros moubata). The optimal infection dosis for jirds was 80 infective larvae (L3). The mean worm number in groups of animals varied between 18 and 30 adult worms. A stable microfilaremia developed and only few animals developed pathological alterations as a consequence of the infection. A simple membrane feeding apparatus allowed mass feeding of ticks. Infection of ticks with microfilariae (mf) using this technique resulted in a mean no. of 594 +/- 527.2 L3/tick. L3 and mf were cryopreserved in liquid N2 with a simple technique. The described maintenance of the life cycle reduced the amount of required experimental animals to 30-40% of the originally needed numbers.