一种从培养物和粪便样本中克隆十二指肠贾第虫的简单方法。
A simple method for cloning Giardia duodenalis from cultures and fecal samples.
作者信息
Binz N, Thompson R C, Meloni B P, Lymbery A J
机构信息
Division of Veterinary Biology, School of Veterinary Studies, Murdoch University, Western Australia.
出版信息
J Parasitol. 1991 Aug;77(4):627-31.
Using a novel method for cloning Giardia duodenalis from cultures and fecal samples, 47 clones from 7 isolates were established in vitro. Average colony-forming efficiency in established cultures was 43.2% compared to 11.2% when cloning directly from excystation. The highest success rate of cloning was found with the Portland (P1, ATCC No. 30888) isolate, with a colony-forming efficiency of 92.7%. Cloned and parent populations were compared over a range of 13 enzymes using starch gel electrophoresis. No genetic difference was found between any of the clones and the parent isolates.
利用一种从培养物和粪便样本中克隆十二指肠贾第虫的新方法，从7个分离株中建立了47个克隆株并进行体外培养。已建立培养物中的平均集落形成效率为43.2%，而直接从脱囊过程中克隆时该效率为11.2%。波特兰（P1，ATCC编号30888）分离株的克隆成功率最高，集落形成效率为92.7%。使用淀粉凝胶电泳对克隆群体和亲本群体在13种酶的范围内进行了比较。未发现任何克隆株与亲本分离株之间存在遗传差异。
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