Isaac-Renton J L, Shahriari H, Bowie W R
Division of Medical Microbiology, University of British Columbia, Vancouver, Canada.
Appl Environ Microbiol. 1992 May;58(5):1530-3. doi: 10.1128/aem.58.5.1530-1533.1992.
An in vitro method and an in vivo method of excystation were compared to determine the most useful method for the retrieval of Giardia duodenalis isolates. Cysts from 11 Giardia strains were used. In vitro excystation produced motile trophozoites in 16 sets, while in vivo excystation produced trophozoites in all of the 21 comparative sets of excystations. Few cultures were lost because of contamination by either method (17% of in vitro-derived trophozoites versus 23% of in vivo-derived trophozoites; P greater than 0.05). Both methods demonstrated comparable isolate retrieval rates (15% of in vitro-derived trophozoites adapting to culture compared with 29% of in vivo-derived trophozoites; P greater than 0.05), although analysis of the strains retrieved showed that two isolates were retrieved from in vitro excystation alone, compared with four from in vivo excystation. Analysis that included results of extra in vivo cultures showed that a total of nine isolates were retrieved by using this type of excystation. Despite the disadvantages of cost and labor, in vivo excystation appears to be more useful than in vitro excystation for isolate retrieval at the present time.
比较了体外脱囊方法和体内脱囊方法，以确定用于获取十二指肠贾第鞭毛虫分离株的最有效方法。使用了来自11株贾第鞭毛虫的包囊。体外脱囊在16组中产生了活动的滋养体，而体内脱囊在所有21组比较脱囊中均产生了滋养体。两种方法因污染而损失的培养物很少（体外来源的滋养体为17%，体内来源的滋养体为23%；P大于0.05）。两种方法的分离株获取率相当（体外来源的滋养体适应培养的比例为15%，体内来源的滋养体为29%；P大于0.05），尽管对获取的菌株分析表明，仅从体外脱囊中获取了2株分离株，而从体内脱囊中获取了4株。包括额外体内培养结果的分析表明，使用这种脱囊类型总共获取了9株分离株。尽管存在成本和劳动力方面的缺点，但目前体内脱囊在分离株获取方面似乎比体外脱囊更有用。
