Carpio Yamila, Lugo Juana María, León Karen, Morales Reynold, Estrada Mario Pablo
Aquatic Biotechnology Department, Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, 10 600, Cuba.
Fish Shellfish Immunol. 2008 Oct;25(4):439-45. doi: 10.1016/j.fsi.2008.06.004. Epub 2008 Jul 3.
There are several studies that clearly indicate a close bidirectional communication between the neuroendocrine and immune systems. In this sense, hypothalamic releasing hormones, besides their neuroendocrine role, have been shown to influence immune functions. Despite studies developed in mammals, there is, as yet, no information available about the role of the pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP-related peptide (PRP) in the fish innate immune system. The present study has evaluated the effect of PACAP and PRP administered by bath immersion, on important parameters of innate immunity and antioxidant defenses in African catfish (Clarias gariepinus) fry. We have shown, for the first time, that administration of recombinant C. gariepinus PACAP not only promotes growth but also increases lysozyme, nitric oxide synthase-derived metabolites and antioxidant defenses in treated fry. From our results, PACAP appears to act as a regulator of the teleostean immune system, in addition to its physiological role in controlling growth of fish.
有多项研究清楚地表明神经内分泌系统与免疫系统之间存在密切的双向通讯。从这个意义上讲，下丘脑释放激素除了具有神经内分泌作用外，还被证明会影响免疫功能。尽管在哺乳动物中开展了相关研究，但目前尚无关于垂体腺苷酸环化酶激活多肽（PACAP）和PACAP相关肽（PRP）在鱼类先天免疫系统中作用的信息。本研究评估了通过浸浴给予PACAP和PRP对非洲鲶鱼（Clarias gariepinus）鱼苗先天免疫和抗氧化防御重要参数的影响。我们首次表明，给予重组的加里波非鲫PACAP不仅能促进生长，还能增加处理后鱼苗的溶菌酶、一氧化氮合酶衍生代谢物和抗氧化防御能力。从我们的结果来看，PACAP除了在控制鱼类生长方面的生理作用外，似乎还充当硬骨鱼免疫系统的调节剂。
