Postaire E, Cisse M, Le Hoang M D, Pradeau D
Pharmaceutical Science University, Malabry, France.
J Pharm Sci. 1991 Apr;80(4):368-70. doi: 10.1002/jps.2600800417.
An over-pressure layer chromatographic procedure with photodensitometric detection for the simultaneous determination of water-soluble vitamins in multivitamin pharmaceutical preparations was developed and evaluated. The method uses high-performance TLC (HPTLC) plates with silica gel as the thin-layer, and an n-butanol:pyridine:water mixture (50:35:15, v/v/v) as mobile phase at a rate of 0.25 mL/min for baseline separation. The quantitation was carried out without derivatization (vitamin B1, vitamin B2, vitamin B6, folic acid, nicotinamide, vitamin C) or after spraying ninhydrin reagent (calcium pantothenate) or 4-dimethylaminocinnamaldehyde (vitamin B12, biotin). This was applied to the analysis of multivitamin solutions. Satisfactory relative standard deviations and good recovery were obtained for all the vitamins examined. It was concluded that this method is fast, accurate, specific, and suitable for routine quality control use.
建立并评估了一种采用光密度检测的超压层色谱法，用于同时测定多种维生素药物制剂中的水溶性维生素。该方法使用以硅胶为薄层的高效薄层色谱（HPTLC）板，以正丁醇：吡啶：水混合物（50:35:15，v/v/v）作为流动相，流速为0.25 mL/min进行基线分离。定量分析在未衍生化的情况下进行（维生素B1、维生素B2、维生素B6、叶酸、烟酰胺、维生素C），或在喷洒茚三酮试剂（泛酸钙）或4-二甲基氨基肉桂醛（维生素B12、生物素）后进行。该方法应用于多种维生素溶液的分析。对于所有检测的维生素，均获得了令人满意的相对标准偏差和良好的回收率。得出的结论是，该方法快速、准确、特异，适用于常规质量控制。
