Shneyour A, Patt Y, Trainin N
J Immunol. 1976 Dec;117(6):2143-9.
Trypsin increases intracellular levels of cylic AMP (cAMP) in lymphocytes. The trypsin-induced increase in cAMP is blocked by specific trypsin inhibitors and by high concentrations of different proteins. Several proteolytic enzymes from various sources, including other pancreatic proteases, do not cause an increase in cAMP under the same experimental conditions. Immobilized trypsin induced the same increase in cAMP as does free trypsin. The trypsin-induced rise in cAMP is not due to inhibition of cAMP phosphodiesterase, but consistent activation of adenylate cyclase by trypsin could not be demonstrated. The extent of the trypsin-induced increase in intracellular cAMP correlates with the type of the lymphocyte and with the state of maturity attained by the cells. Transformed lymphocytes and nonlymphoid cells do not react at all.
胰蛋白酶可提高淋巴细胞内环状AMP（cAMP）的水平。胰蛋白酶诱导的cAMP增加可被特异性胰蛋白酶抑制剂和高浓度的不同蛋白质所阻断。在相同实验条件下，来自各种来源的几种蛋白水解酶，包括其他胰腺蛋白酶，不会导致cAMP增加。固定化胰蛋白酶诱导的cAMP增加与游离胰蛋白酶相同。胰蛋白酶诱导的cAMP升高并非由于抑制了cAMP磷酸二酯酶，但未能证明胰蛋白酶能持续激活腺苷酸环化酶。胰蛋白酶诱导的细胞内cAMP增加程度与淋巴细胞类型以及细胞达到的成熟状态相关。转化淋巴细胞和非淋巴细胞根本没有反应。
