Ulrich F
Immunology. 1979 Dec;38(4):705-15.
When rat thymocytes were cultured with concanavalin A (con A), addition of trypsin increased the incorporation of [H]-thymidine (H-TdR) several fold over that observed with mitogen alone. The potentiating effect of trypsin was greatest with suboptimal concentrations of mitogen, less with optimal, and least with superoptimal concentrations. Trypsin also increased blastogenesis and stimulated [H]-leucine incorporation into thymocyte proteins. Thymocytes cultured with trypsin in the absence of mitogen sometimes showed increased H-TdR incorporation but the increase was usually small and not consistent. The potentiating action of trypsin is due to its proteolytic activity since it was completely blocked by soybean trypsin inhibitor. Elastase, α-chymotrypsin, and subtilisin also potentiated the action of con A, while papain, collagenase, thrombin, and plasmin had either very little or no effect. Trypsin potentiated H-TdR incorporation into thymocytes cultured in serum-free or serum-supplemented media, but its effects were usually greater in media supplemented with 5% autologous serum. Although heating serum at 56° for 0.5 or 2 h markedly reduced stimulation by con A, the effects of trypsin were greater in media supplemented with heated serum. The possible physiological significance of the results was discussed with respect to protease secretion by macrophages and lymphocyte—macrophage interactions.
当用伴刀豆球蛋白A（Con A）培养大鼠胸腺细胞时，添加胰蛋白酶能使[³H] - 胸腺嘧啶核苷（³H - TdR）的掺入量比单独使用促有丝分裂原时增加几倍。胰蛋白酶的增强作用在促有丝分裂原浓度次优时最大，在最优浓度时较小，在超优浓度时最小。胰蛋白酶还能增加细胞分裂增殖，并刺激[³H] - 亮氨酸掺入胸腺细胞蛋白。在无促有丝分裂原的情况下用胰蛋白酶培养的胸腺细胞有时显示³H - TdR掺入量增加，但增加通常较小且不一致。胰蛋白酶的增强作用归因于其蛋白水解活性，因为它被大豆胰蛋白酶抑制剂完全阻断。弹性蛋白酶、α - 糜蛋白酶和枯草杆菌蛋白酶也能增强Con A的作用，而木瓜蛋白酶、胶原酶、凝血酶和纤溶酶的作用很小或没有作用。胰蛋白酶能增强³H - TdR掺入无血清或添加血清培养基中培养的胸腺细胞，但在添加5%自体血清的培养基中其作用通常更大。虽然将血清在56℃加热0.5或2小时会显著降低Con A的刺激作用，但在添加加热血清的培养基中胰蛋白酶的作用更大。针对巨噬细胞分泌蛋白酶和淋巴细胞 - 巨噬细胞相互作用讨论了这些结果可能的生理学意义。
