Joshi Amit, Punyani Supriya, Bale Shyam Sundhar, Yang Hoichang, Borca-Tasciuc Theodorian, Kane Ravi S
Nat Nanotechnol. 2008 Jan;3(1):41-5. doi: 10.1038/nnano.2007.386. Epub 2007 Dec 9.
Conjugating proteins onto carbon nanotubes has numerous applications in biosensing, imaging and cellular delivery. However, remotely controlling the activity of proteins in these conjugates has never been demonstrated. Here we show that upon near-infrared irradiation, carbon nanotubes mediate the selective deactivation of proteins in situ by photochemical effects. We designed nanotube-peptide conjugates to selectively destroy the anthrax toxin, and also optically transparent coatings that can self-clean following either visible or near-infrared irradiation. Nanotube-assisted protein deactivation may be broadly applicable to the selective destruction of pathogens and cells, and will have applications ranging from antifouling coatings to functional proteomics.
将蛋白质与碳纳米管结合在生物传感、成像和细胞递送方面有众多应用。然而，远程控制这些结合物中蛋白质的活性从未得到证实。在此我们表明，在近红外照射下，碳纳米管通过光化学效应介导蛋白质原位选择性失活。我们设计了纳米管 - 肽结合物以选择性地破坏炭疽毒素，还设计了在可见光或近红外照射后可自清洁的光学透明涂层。纳米管辅助的蛋白质失活可能广泛适用于病原体和细胞的选择性破坏，并将在从防污涂层到功能蛋白质组学等领域有应用。
