Fu Xin, Lin Lin, Zhang Jiying, Yu Changlong
Institute of Sports Medicine, Peking University Third Hospital, Beijing, People's Republic of China.
J Orthop Res. 2009 Jan;27(1):91-6. doi: 10.1002/jor.20720.
We investigated the efficacy of joint lavage to alter the progression of the disease process in a rabbit model of knee OA in varying stages of the disease. Thirty-three white rabbits were operated to induce OA, and then the rabbits were divided into three groups (10 in each) randomly. In group 1, on week 1 after the operation, joint lavage was performed on both knees in five rabbits as treatment group, and the other five rabbits were used as control group received no intervention. By the end of week 2 after operation, the rabbits were sacrificed. In group 2, five received joint lavage on week 2, and all were sacrificed on week 3. In group3, five received joint lavage on week 3, and all were sacrificed on week 4. In each group, histological evaluation showed that both the breakdown of articular cartilage and the inflammation of synovium were less in the knees treated with joint lavage than that in the control knees. The enzyme-linked immunosorbent assay revealed that the expression of IL-1beta and TNF-alpha in synovial fluid decreased significantly in the treatment group. Our findings suggested that joint lavage was beneficial for OA at different phases of OA in rabbit models. Joint lavage may be a beneficial method for the treatment of OA clinically.
我们在处于不同疾病阶段的兔膝骨关节炎模型中，研究了关节灌洗改变疾病进程的疗效。33只白兔接受手术诱导骨关节炎，然后将这些兔子随机分为三组（每组10只）。在第1组中，术后第1周，对5只兔子的双膝进行关节灌洗作为治疗组，另外5只兔子作为对照组不接受干预。术后第2周结束时，处死兔子。在第2组中，5只兔子在第2周接受关节灌洗，所有兔子在第3周处死。在第3组中，5只兔子在第3周接受关节灌洗，所有兔子在第4周处死。在每组中，组织学评估显示，接受关节灌洗的膝关节的关节软骨破坏和滑膜炎症均比对照膝关节轻。酶联免疫吸附测定显示，治疗组滑液中IL-1β和TNF-α的表达显著降低。我们的研究结果表明，在兔模型中，关节灌洗对骨关节炎不同阶段均有益。关节灌洗可能是临床上治疗骨关节炎的一种有益方法。
