Crow Brian, Bishop Michael, Paliakov Ekaterina, Norton Dean, George Joe, Bralley J Alexander
Metametrix Clinical Laboratory, 3425 Corporate Way, Duluth, GA 30096, USA.
Biomed Chromatogr. 2008 Dec;22(12):1346-53. doi: 10.1002/bmc.1064.
The separation and detection of 11 urinary aromatic acids was developed using HPLC-MS/MS. The method features a simple sample preparation involving a single-step dilution with internal standard and a rapid 8 min chromatographic separation. The accuracy was evaluated by the recovery of known spikes between 87 and 110%. Inter- and intra-assay precision (CV) was below 11% in all cases and the analytes were observed to be stable for up to 8 weeks when stored at -20 degrees C. The method was validated based upon linearity, accuracy, precision and stability and was used to establish reference intervals for children and adults.
采用高效液相色谱-串联质谱法（HPLC-MS/MS）对11种尿中芳香酸进行分离和检测。该方法的特点是样品制备简单，只需用内标进行一步稀释，色谱分离快速，8分钟即可完成。通过回收率评估准确度，已知加标回收率在87%至110%之间。批间和批内精密度（CV）在所有情况下均低于11%，并且观察到分析物在-20℃储存时长达8周都是稳定的。该方法基于线性、准确度、精密度和稳定性进行了验证，并用于建立儿童和成人的参考区间。
