抗干扰素血清在小鼠病毒病发病机制中对干扰素作用的研究。I. 脑心肌炎病毒感染的快速演变
Role of interferon in the pathogenesis of virus diseases in mice as demonstrated by the use of anti-interferon serum. I. Rapid evolution of encephalomyocarditis virus infection.
作者信息
Gresser I, Tovey M G, Bandu M E, Maury C, Brouty-Boyé D
出版信息
J Exp Med. 1976 Nov 2;144(5):1305-15. doi: 10.1084/jem.144.5.1305.
The role of interferon in the pathogenesis of encephalomyocarditis (EMC) virus infection was determined by treating mice with potent, partially purified sheep anti-mouse interferon globulin. In control mice, EMC virus was present in low titers in various visceral organs but attained high titers in the brain towards the 4th to 5th day, at which time mice died with signs of central nervous system disease. In mice treated with anti-mouse interferon globulin, virus was present in high titer in visceral organs 24--36 h after viral inoculation and virtually all mice were dead by 45 h. This rapid evolution of EMC virus infection was not observed in mice treated with the globulin fraction prepared from a normal sheep, from a sheep exhibiting a low anti-mouse interferon-neutralizing titer, nor from a sheep having a high titer of antibody to human leukocyte interferon. The experimental results indicated that anti-interferon globulin neutralized the interferon liberated by virus-infected cells, thus permitting extensive virus multiplication in several visceral organs. We conclude that interferon is an important early component of host resistance to this virus infection.
通过用高效、部分纯化的羊抗小鼠干扰素球蛋白处理小鼠，确定了干扰素在脑心肌炎（EMC）病毒感染发病机制中的作用。在对照小鼠中，EMC病毒在各种内脏器官中的滴度较低，但在第4至5天时在脑中达到高滴度，此时小鼠死于中枢神经系统疾病的症状。在用抗小鼠干扰素球蛋白处理的小鼠中，病毒接种后24至36小时内脏器官中病毒滴度很高，几乎所有小鼠在45小时内死亡。在用从正常绵羊、抗小鼠干扰素中和滴度低的绵羊或对人白细胞干扰素有高滴度抗体的绵羊制备的球蛋白部分处理的小鼠中，未观察到EMC病毒感染的这种快速演变。实验结果表明，抗干扰素球蛋白中和了病毒感染细胞释放的干扰素，从而允许病毒在几个内脏器官中大量繁殖。我们得出结论，干扰素是宿主抵抗这种病毒感染的重要早期成分。
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