Jin Dazhi, Qi Hongjuan, Chen Suhong, Zeng Ting, Liu Qiqi, Wang Shengqi
Beijing Institute of Radiation Medicine, No. 27 Taiping road, Beijing, China.
J Microbiol Methods. 2008 Oct;75(2):365-8. doi: 10.1016/j.mimet.2008.06.020. Epub 2008 Jul 2.
Multiplex PCR and DNA microarray were combined with tyramide signal amplification (TSA) to develop a reliable method suitable for simultaneous detection of six species of human diarrheal pathogens (Yersinia enterocolitica, Shigella spp, Salmonella typhi, Brucella spp, Vibrio cholera and Escherichia coli O157:H7). Meanwhile, our method could distinguish V. cholera serotype O1 from O139, and O157:H7 from O157: non-H7. This assay conferred a specificity of 100% for target pathogens. The limit of detection was 103 degrees CFU/mL approximately. The results of 98.6% (357/362) clinical specimens and 100% (5/5) mocked double-blind samples were the same to that from conventional assay. Consequently this assay is sensitive and a specific tool suitable for diagnostic detection and surveillance of multiple human pathogens.
多重聚合酶链反应（Multiplex PCR）和DNA微阵列与酪胺信号放大（TSA）相结合，开发出一种可靠的方法，适用于同时检测六种人类腹泻病原体（小肠结肠炎耶尔森菌、志贺菌属、伤寒沙门菌、布鲁氏菌属、霍乱弧菌和大肠杆菌O157:H7）。同时，我们的方法可以区分霍乱弧菌O1血清型和O139血清型，以及O157:H7和O157:非H7。该检测方法对目标病原体的特异性为100%。检测限约为103CFU/mL。98.6%（357/362）临床标本和100%（5/5）模拟双盲样本的检测结果与传统检测方法相同。因此，该检测方法灵敏且特异，是适用于多种人类病原体诊断检测和监测的工具。
