Division of Biomedical Research, Sri Narayani Hospital and Research Centre, Thirumalaikodi, Sripuram, Vellore, Tamil Nadu, India.
Mol Diagn Ther. 2011 Jun 1;15(3):145-58. doi: 10.1007/BF03256405.
Infectious diseases are a major global public health problem. Multiple agents are now recognized to cause indistinguishable illnesses. The term 'syndrome' applies to such situations, for which early and rapid diagnosis of the infecting agent would enable prompt and appropriate therapy. Public health physicians would also get timely information on the specific etiology of the infectious syndrome, facilitating intervention at the community level in the face of outbreaks or epidemics. A variety of molecular techniques have been evaluated for rapid diagnosis of infectious syndromes. These techniques include real-time multiplex PCR, DNA microarray, loop-mediated isothermal amplification, and other similar assays. This review surveys such state-of-the-art technologies.
传染病是一个全球性的主要公共卫生问题。现在已经认识到多种病原体可引起无法区分的疾病。“综合征”一词适用于这种情况，对于这种情况，早期和快速诊断病原体将能够及时进行适当的治疗。公共卫生医生也将及时获得有关传染病综合征具体病因的信息，以便在面对疫情或流行时在社区层面进行干预。已经评估了多种分子技术来快速诊断传染病综合征。这些技术包括实时多重 PCR、DNA 微阵列、环介导等温扩增和其他类似的检测方法。本综述调查了这些最先进的技术。
