Wu Lina, Lei Jianping, Zhang Xueji, Ju Huangxian
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, PR China.
Biosens Bioelectron. 2008 Dec 1;24(4):644-9. doi: 10.1016/j.bios.2008.06.009. Epub 2008 Jun 17.
A biofunctional hybrid nanocomposite of carbon nanofiber (CNF) with water-soluble iron(III) meso-tetrakis(N-methylpyridinum-4-yl) porphyrin (FeTMPyP) was designed via non-covalent interaction for preparation of highly sensitive ethanol biosensor. The prepared nanocomposite showed good dispersion in water and was characterized with steady-state electronic absorption spectroscopy and scanning electron microscope. The nanocomposite combined the good conductivity of CNF and the excellent catalytic activity of both CNF and FeTMPyP toward the reduction of dissolved oxygen, producing a method for amperometric detection of oxygen ranging from 6.5 nM to 6.4 microM at a low overpotential. The nanocomposite modified electrode was further used for assembly of alcohol oxidase to construct an amperometric biosensor for ethanol. The biosensor showed rapid and highly sensitive response to ethanol with a linear range from 2.0 microM to 112 microM. The immobilized alcohol oxidase also showed its direct electrochemistry. The biofunctional nanocomposite provides a new way to not only construct the highly sensitive biosensors but also mimic the catalytic activity of enzyme in the life process.
通过非共价相互作用设计了一种碳纳米纤维（CNF）与水溶性铁（III）中-四（N-甲基吡啶-4-基）卟啉（FeTMPyP）的生物功能杂化纳米复合材料，用于制备高灵敏度乙醇生物传感器。所制备的纳米复合材料在水中表现出良好的分散性，并通过稳态电子吸收光谱和扫描电子显微镜进行了表征。该纳米复合材料结合了CNF的良好导电性以及CNF和FeTMPyP对溶解氧还原的优异催化活性，产生了一种在低过电位下对6.5 nM至6.4 μM范围内的氧气进行安培检测的方法。该纳米复合材料修饰电极进一步用于组装醇氧化酶，以构建用于乙醇的安培生物传感器。该生物传感器对乙醇表现出快速且高度灵敏的响应，线性范围为2.0 μM至112 μM。固定化的醇氧化酶也显示出其直接电化学性质。这种生物功能纳米复合材料不仅为构建高灵敏度生物传感器提供了一种新方法，而且还能模拟生命过程中酶的催化活性。
