Wu Lina, Zhang Xueji, Ju Huangxian
MOE Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, P.R. China.
Anal Chem. 2007 Jan 15;79(2):453-8. doi: 10.1021/ac061282+.
The excellent catalytic activity of a novel carbon nanomaterial, soluble carbon nanofiber (CNF) with good dispersion and wettability, to the oxidation of dihydronicotinamide adenine dinucleotide (NADH) was described for biosensing application. The edge plane sites and oxygen-rich groups presented on the CNF surface could be partially responsible for its electrocatalytic behavior, which induced a substantial decrease by 573 mV in the overpotential of NADH oxidation reaction (compared to a bare electrode). The CNF-coated electrode thus allowed highly sensitive amperometric detection of NADH with a low limit of detection (0.11 muM), low applied potential (+0.06 V), and minimization of surface fouling. Such ability of CNF to promote the electron transfer between NADH and the electrode suggested a new, promising biocompatible platform for development of dehydrogenase-based amperometric biosensors. With alcohol dehydrogenase (ADH) as a model, the ADH/CNF-modified electrode could be constructed by a simple casting process. The proposed biosensor showed rapid and highly sensitive amperometric response to ethanol with acceptable preparation reproducibility and excellent stability.
一种新型碳纳米材料——具有良好分散性和润湿性的可溶性碳纳米纤维（CNF），对二氢烟酰胺腺嘌呤二核苷酸（NADH）氧化具有优异的催化活性，可用于生物传感应用。CNF表面存在的边缘平面位点和富氧基团可能部分归因于其电催化行为，这使得NADH氧化反应的过电位大幅降低了573 mV（与裸电极相比）。因此，涂有CNF的电极能够以低检测限（0.11 μM）、低施加电位（+0.06 V）以及最小化表面污染实现对NADH的高灵敏度安培检测。CNF促进NADH与电极之间电子转移的这种能力为基于脱氢酶的安培生物传感器的开发提供了一个新的、有前景的生物相容性平台。以乙醇脱氢酶（ADH）为模型，可通过简单的浇铸工艺构建ADH/CNF修饰电极。所提出的生物传感器对乙醇表现出快速且高度灵敏的安培响应，具有可接受的制备重现性和出色的稳定性。
