Kim Ekyune, Lee Jae-Woong, Baek Dong-Chul, Lee Sang-Rae, Kim Myeong-Su, Kim Sang-Hyun, Imakawa Kazuhiko, Chang Kyu-Tae
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, 685-1 Yangcheong-ri, Ochang-eup, Chung-buk 363-883, Republic of Korea.
Biochem Biophys Res Commun. 2008 Oct 10;375(1):16-21. doi: 10.1016/j.bbrc.2008.07.067. Epub 2008 Jul 24.
A family of vacuolar protein sorting (Vps) proteins, which are components of mammalian retromer complex, has been studied in the mouse. Vps26a is known as a retromer component that plays an important role in embryonic development: however, its cell-type expression and precise role remain to be elucidated. In this study, we identified a new isoform of Vps26a, called Vps26aT, which was expressed specifically in the mouse testis. Diverse expression patterns of Vps26 variants in mouse tissues were determined by Western blot and RT-PCR analyses, and the direct interaction of Vps26aT with Vps35 was also demonstrated by immunoprecipitation and pull-down assay using antibodies raised against each Vps component. Our results revealed that the retromer complex could be formed from different Vps26 isoforms in a tissue-specific manner, resulting in more than two types of the retromer complex, including the Vps26a-Vps29-Vps35, Vps26aT-Vps29-Vps35, and Vps26b-Vps29-Vps35 complexes in mouse tissues.
一类液泡蛋白分选（Vps）蛋白作为哺乳动物逆转录复合物的组成部分，已在小鼠中得到研究。Vps26a是一种已知的逆转录复合物成分，在胚胎发育中起重要作用：然而，其细胞类型表达和确切作用仍有待阐明。在本研究中，我们鉴定出一种新的Vps26a亚型，称为Vps26aT，它在小鼠睾丸中特异性表达。通过蛋白质免疫印迹和逆转录聚合酶链反应（RT-PCR）分析确定了Vps26变体在小鼠组织中的不同表达模式，并且还通过使用针对每种Vps成分产生的抗体进行免疫沉淀和下拉试验，证明了Vps26aT与Vps35的直接相互作用。我们的结果表明，逆转录复合物可以由不同的Vps26亚型以组织特异性方式形成，从而在小鼠组织中产生两种以上类型的逆转录复合物，包括Vps26a-Vps29-Vps35、Vps26aT-Vps29-Vps35和Vps26b-Vps29-Vps35复合物。
