Fuse Atsuhito, Furuya Norihiko, Kakuta Soichiro, Inose Aki, Sato Masumi, Koike Masato, Saiki Shinji, Hattori Nobutaka
Department of Neurology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.
Department of Neurology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan; Department of Research and Therapeutics for Movement Disorders, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.
FEBS Lett. 2015 Jun 4;589(13):1430-6. doi: 10.1016/j.febslet.2015.04.040. Epub 2015 May 1.
Retromer is a complex of proteins that functions in the endosome-to-Golgi retrieval cargo transport pathway. VPS35 works as the central subunit of retromer to recognize the cargos and binds with VPS29 and VPS26 via distinct domains. We show that deficiency of VPS35 or VPS29 accompanies degradation of other subunits, whereas VPS26 deficiency had no effect on VPS29 and VPS35 levels. Although VPS35 forms VPS26-VPS35 and VPS29-VPS35 sub-complexes with similar efficiency in vitro, VPS26-VPS35 was more easily degradable by the ubiquitin-proteasome-system than VPS29-VPS35. These results indicate that VPS29 and VPS35 form a biologically stable sub-complex in vivo.
回收体是一种蛋白质复合体，在从内体到高尔基体的货物回收转运途径中发挥作用。VPS35作为回收体的核心亚基，识别货物并通过不同结构域与VPS29和VPS26结合。我们发现，VPS35或VPS29的缺失会伴随着其他亚基的降解，而VPS26的缺失对VPS29和VPS35的水平没有影响。尽管VPS35在体外以相似的效率形成VPS26-VPS35和VPS29-VPS35亚复合体，但与VPS29-VPS35相比，VPS26-VPS35更容易被泛素-蛋白酶体系统降解。这些结果表明，VPS29和VPS35在体内形成了一种生物学上稳定的亚复合体。
