Jaatinen S T, Happonen L J, Laurinmäki P, Butcher S J, Bamford D H
Department of Biological and Environmental Sciences and Institute of Biotechnology, Biocenter 2, FIN-00014, University of Helsinki, Finland.
Virology. 2008 Sep 15;379(1):10-9. doi: 10.1016/j.virol.2008.06.023. Epub 2008 Jul 25.
Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection. The structural protein patterns of three of these viruses, growing to a high titer, appeared very similar but not identical. The most stable virus, P23-77, was chosen for more detailed studies. Analysis of highly purified P23-77 by thin layer chromatography for neutral lipids showed lipid association with the virion. Cryo-EM based three-dimensional image reconstruction of P23-77 to 1.4 nm resolution revealed an icosahedrally-ordered protein coat, with spikes on the vertices, and an internal membrane. The capsid architecture of P23-77 is most similar to that of the archaeal virus SH1. These findings further complicate the grouping of icosahedrally-symmetric viruses containing an inner membrane. We propose a single superfamily or order with members in several viral families.
从温泉中分离出的、被认为属于复层噬菌体科的二十面体双链DNA病毒可感染革兰氏阴性嗜热栖热菌。从普洛麦格公司的样本库中获得了七种这样的病毒。其中三种病毒生长到高滴度时，其结构蛋白模式看起来非常相似，但并不完全相同。选择最稳定的病毒P23 - 77进行更详细的研究。通过薄层层析对高度纯化的P23 - 77进行中性脂质分析，结果显示脂质与病毒粒子有关联。基于冷冻电镜的P23 - 77三维图像重建至1.4纳米分辨率，揭示出一个二十面体有序的蛋白质外壳，在顶点处有刺突，还有一层内膜。P23 - 77的衣壳结构与古病毒SH1的最为相似。这些发现进一步使含有内膜的二十面体对称病毒的分类变得复杂。我们提出一个单一的超家族或目，其中有几个病毒科的成员。
