Wang Zhiping, Zhang Shicui, Wang Guangfeng
Department of Marine Biology, Ocean University of China, Qingdao, PR China.
Fish Shellfish Immunol. 2008 Sep;25(3):264-70. doi: 10.1016/j.fsi.2008.05.010. Epub 2008 Jul 25.
The development of complement in fish is poorly understood, and little information is available about the acquisition of full complement capacities. In this study we demonstrated the ontogenic and differential expression of complement key component genes (C3, C1r/s, C4, C6, Bf, MBL and MASP) of the classical pathway (CP), alternative pathway (AP), lectin pathway (LP) and lytic pathway, and their responses to challenge with LPS during development. All components, except C6, showed a similar trend of increasing expression after hatching. The contents of Bf and C3 transcripts were both markedly increased by challenge with LPS soon after hatching, while the expression levels of C1r/s and MASP transcripts were not elevated. The expression of C4 mRNA was first down-regulated by LPS challenge and then fluctuated. The expression of MBL mRNA was up-regulated by LPS challenge after 18 days post fertilization. In response to LPS challenge, C6 was peculiarly expressed: it was significantly decreased before hatching, followed by a significant up-regulation post hatching. Taken together, these suggest that the complement operating via the AP is already competent by responding to challenge with LPS in the hatched larvae of Danio rerio.
鱼类补体系统的发育情况鲜为人知，关于其完整补体能力的获得也知之甚少。在本研究中，我们展示了经典途径（CP）、替代途径（AP）、凝集素途径（LP）和溶解途径的补体关键成分基因（C3、C1r/s、C4、C6、Bf、MBL和MASP）的个体发育及差异表达，以及它们在发育过程中对脂多糖（LPS）刺激的反应。除C6外，所有成分在孵化后均呈现出相似的表达增加趋势。孵化后不久，LPS刺激显著增加了Bf和C3转录本的含量，而C1r/s和MASP转录本的表达水平并未升高。LPS刺激首先下调了C4 mRNA的表达，随后其表达出现波动。受精后18天，LPS刺激上调了MBL mRNA的表达。对LPS刺激的反应中，C6的表达较为特殊：孵化前显著下降，孵化后则显著上调。综上所述，这些结果表明，斑马鱼孵化后的幼体通过替代途径起作用的补体已经能够对LPS刺激作出反应。
