Schmued Larry, Bowyer John, Cozart Matthew, Heard David, Binienda Zbigniew, Paule Merle
Division of Neurotoxicology, National Center for Toxicological Research/FDA, Jefferson AR 72079, USA.
Brain Res. 2008 Sep 10;1229:210-7. doi: 10.1016/j.brainres.2008.06.129. Epub 2008 Jul 15.
A novel gold phosphate complex called Black-Gold II with improved myelin staining properties has been developed. It differs from its predecessor, Black-Gold, in that it is highly water soluble at room temperature. This unique physical property confers a number of advantages for the high resolution staining of myelinated fibers. Specifically, it 1) allows for easier solution preparation, eliminating the need for extended heating or sonicating; 2) produces a more uniform and consistent tracer concentration, resulting in more consistent staining and 3) can be used at a 50% higher concentration, resulting in faster and more intense staining without the need for subsequent treatment with gold chloride intensifiers. To characterize the stain, both normal rat brains as well as those exposed to the neurotoxins kainic acid or methamphetamine were examined. The study also incorporates the first application of such stains to examine peripheral nerves of control and acrylamide-exposed rats.
一种名为黑金II的新型磷酸金复合物已被开发出来，其髓磷脂染色特性得到了改善。它与前身黑金的不同之处在于，它在室温下具有高度水溶性。这种独特的物理特性为有髓纤维的高分辨率染色带来了许多优势。具体而言，它1）使溶液制备更容易，无需长时间加热或超声处理；2）产生更均匀一致的示踪剂浓度，从而实现更一致的染色；3）可以以高50%的浓度使用，无需后续用氯化金增强剂处理即可实现更快、更强的染色。为了对这种染色剂进行表征，研究人员检查了正常大鼠大脑以及暴露于神经毒素海藻酸或甲基苯丙胺的大鼠大脑。该研究还首次应用此类染色剂来检查对照大鼠和接触丙烯酰胺大鼠的外周神经。
