Schmued L, Slikker W
Division of Neurotoxicology, National Center for Toxicological Research/FDA, 3900 NCTR Road, Jefferson, AR 72079-9502, USA.
Brain Res. 1999 Aug 7;837(1-2):289-97. doi: 10.1016/s0006-8993(99)01624-8.
A novel haloaurophosphate complex called Black-Gold has been synthesized and applied to localize myelin within the central nervous system. The technique is tailored to studies using formalin fixed non-solvent processed tissue. The technique stains large myelinated tracts dark red-brown, while the individual myelinated axons appear black. This study demonstrates how this novel tracer can be used to localize both normal and pathological myelin. Specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid are demonstrated and characterized. This study also demonstrates how Black-Gold can be combined with other histochemical markers including Nissl stains, retrogradely transported fluorescent tracers and fluorescent markers of neuronal degeneration. Advantages associated with the Black-Gold technique include high resolution, high contrast, short histochemical processing time, and consistent reproducibility.
一种名为“黑金”的新型卤金磷酸盐复合物已被合成，并应用于中枢神经系统内髓磷脂的定位。该技术适用于使用福尔马林固定、非溶剂处理组织的研究。该技术将大的有髓神经束染成深红褐色，而单个有髓轴突则呈现黑色。这项研究展示了这种新型示踪剂如何用于定位正常和病理性髓磷脂。研究证明并描述了与接触多种神经毒素（包括 kainic 酸、软骨藻酸、3-硝基丙酸、荧光金和异烟肼）相关的特定髓磷脂变化。这项研究还展示了“黑金”如何与其他组织化学标记物（包括尼氏染色、逆行运输的荧光示踪剂和神经元变性的荧光标记物）相结合。“黑金”技术的优点包括高分辨率、高对比度、短组织化学处理时间和一致的可重复性。
