Stolte Ellen H, Savelkoul Huub F J, Wiegertjes Geert, Flik Gert, Lidy Verburg-van Kemenade B M
Cell Biology and Immunology Group, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands.
Dev Comp Immunol. 2008;32(12):1467-81. doi: 10.1016/j.dci.2008.06.012. Epub 2008 Jul 25.
Two interferon gamma (IFN-gamma) genes are expressed in immune cells of teleost fish and are potentially implicated in B- and T-lymphocyte responses. IFN-gamma-2 shows structural and functional characteristics to other vertebrate IFN-gamma genes and is associated with T-lymphocyte function. Expression profiling shows IFN-gamma-2 upregulation in T-lymphocytes after phytohemagglutinin (PHA) stimulation in vitro. Unexpectedly, we found IFN-gamma-1, which is structurally different from IFN-gamma-2, to be expressed in lipopolysacharide (LPS)-stimulated IgM+ (B- lymphocyte enriched) fractions. Expression of T-box transcription factor T-bet, but not of GATA-binding protein 3 (GATA3), correlated with expression of both IFN-gamma genes. In-vivo parasite infection, but as predicted not zymosan-induced inflammation, resulted in concomitant upregulation of T-bet and IFN-gamma-2. This corroborates a genuine T-lymphocyte associated role for IFN-gamma-2.
硬骨鱼的免疫细胞中表达两种干扰素γ（IFN-γ）基因，它们可能与B淋巴细胞和T淋巴细胞反应有关。IFN-γ-2与其他脊椎动物的IFN-γ基因具有结构和功能特征，并且与T淋巴细胞功能相关。表达谱显示，在体外经植物血凝素（PHA）刺激后，T淋巴细胞中IFN-γ-2上调。出乎意料的是，我们发现结构与IFN-γ-2不同的IFN-γ-1在脂多糖（LPS）刺激的IgM+（富含B淋巴细胞）组分中表达。T盒转录因子T-bet而非GATA结合蛋白3（GATA3）的表达与两种IFN-γ基因的表达相关。体内寄生虫感染而非如预期的酵母聚糖诱导的炎症导致T-bet和IFN-γ-2同时上调。这证实了IFN-γ-2在真正的T淋巴细胞相关作用。
