Gutierrez Rosa Martha Perez, Solis Rosario Vargas
Laboratorio de Investigación de Productos Naturales, Escuela Superior de Ingenierïa Quïmica e Industrias Extractivas IPN, Av. Otón de Mendizábal S/N Unidad Profesional Adolfo López Mateos, Mexico D.F., Mexico.
J Nat Med. 2009 Jan;63(1):65-8. doi: 10.1007/s11418-008-0280-x. Epub 2008 Jul 31.
The antispasmodic effects of hexane-, chloroform-, methanol- and water-extracts of the orchid Encyclia michuacana were studied in vitro on guinea pig ileum against three spasmogens: acetylcholine (Ach), histamine and barium chloride. The chloroform extract exerted a significant antispasmodic effect on ileum contractions induced by Ach, histamine and barium chloride (IC(50) = 90.64, 73.12 and 115.2 microg/mL, respectively). Furthermore, the chloroform extract of E. michuacana provoked a concentration-dependent inhibition of spontaneous contractions of guinea pig ileum with potencies comparable to those of papaverine. The antagonism against the spasmogens used suggests that the action of the chloroform extract of E. michuacana could be due mainly to the presence of gigantol. Hexane-, methanol- and water-extracts did not elicit any significant spasmolytic activity.
研究了米却肯树兰的己烷、氯仿、甲醇和水提取物在体外对豚鼠回肠针对三种致痉剂（乙酰胆碱、组胺和氯化钡）的解痉作用。氯仿提取物对由乙酰胆碱、组胺和氯化钡诱导的回肠收缩具有显著的解痉作用（IC(50)分别为90.64、73.12和115.2微克/毫升）。此外，米却肯树兰的氯仿提取物引起豚鼠回肠自发收缩的浓度依赖性抑制，其效力与罂粟碱相当。对所用致痉剂的拮抗作用表明，米却肯树兰氯仿提取物的作用可能主要归因于巨球兰酚的存在。己烷、甲醇和水提取物未表现出任何显著的解痉活性。
