Furukawa Kazuhiro, Abe Hiroshi, Abe Naoko, Harada Mitsuru, Tsuneda Satoshi, Ito Yoshihiro
Nano Medical Engineering Laboratory, Advanced Science Institute, RIKEN 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
Bioorg Med Chem Lett. 2008 Aug 15;18(16):4562-5. doi: 10.1016/j.bmcl.2008.07.040. Epub 2008 Jul 15.
We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.
我们展示了利用滚环转录法生成孔雀石绿（MG）RNA适体的串联重复序列。为了在长RNA上保持每个适体的高级结构，我们设计了一个带有14个碱基连接子的环状DNA序列。在MG RNA适体的特异性转录方面，T7 RNA聚合酶优于大肠杆菌RNA聚合酶。最后，证实了带有MG的适体重复序列产生了荧光信号。
